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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Changes in colonic contractility in response to inflammatory bowel disease: Long-term assessment in a model of TNBS-induced inflammation in rats

Texto completo
Autor(es):
Calabresi, Marcos F. F. [1] ; Tanimoto, Alexandre [2] ; Prospero, Andre G. [1] ; Mello, Fabio P. F. [1] ; Soares, Guilherme [1] ; Di Stasi, Luiz C. [2] ; Miranda, Jose R. A. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, Biosci Inst, Dept Phys & Biophys, Lab Biomagnetism, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Biosci Inst, Dept Pharmacol, Lab Phytomed Pharmacol & Biotechnol, Botucatu, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Life Sciences; v. 236, NOV 1 2019.
Citações Web of Science: 0
Resumo

Aims: Inflammatory bowel disease is a chronic relapsing inflammation that affects the gastrointestinal tract, causing changes in colonic motility. The evolution of these changes is not completely understood and possibly related to symptoms that appear in different degrees of the intestinal inflammation. Therefore, our aim is evaluate during 14 days of assessment aspects of colonic contractility using 2,4,6-trinitrobenzenesulfonic acid (TNBS) model of inflammation in rats and associate the inflammatory process with colonic motility. Methods: Contractility and inflammatory parameters were assessed in the same animal in six different moments: before intestinal inflammation induction, 2, 5, 8, 11, and 14 days after induction. The mechanical activity was determined by alternating current biosusceptometry (ACB) and subdivided into rhythmic propagating ripples (RPR) and rhythmic propulsive motor complexes (RPMC). We assessed inflammation by determining myeloperoxidase activity in feces. Results: Transient and permanent changes were observed in colonic motility as a function of the inflammatory process evaluated through myeloperoxidase activity. We identified two contraction profiles: RPR and RPMC. The microscopic analysis demonstrated a depth of damage caused by an injury that was associated with changes in motility. Conclusions: We implemented a robust and adequate (specific) signal processing to quantify two measured colonic frequency patterns. Thus, we performed a detailed temporal analysis of the consequences of TNBS-induced inflammation on colonic motility in rats. Our approach enables further long-term assessments in the same animal with different mechanisms and duration of injury, remission, treatments and their motor consequences. (AU)

Processo FAPESP: 13/09095-4 - Estudo elétrico e mecânico da motilidade colônica sob influencia de colite ulcerativa
Beneficiário:Marcos Felipe de Freitas Calabresi
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 15/14923-9 - Instrumentação e aplicações por biosusceptometria de corrente alternada: estudos de distribuição de nanopartículas, motilidade e controle da qualidade de formas farmacêuticas sólidas
Beneficiário:José Ricardo de Arruda Miranda
Linha de fomento: Auxílio à Pesquisa - Regular