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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Changes in colonic contractility in response to inflammatory bowel disease: Long-term assessment in a model of TNBS-induced inflammation in rats

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Author(s):
Calabresi, Marcos F. F. [1] ; Tanimoto, Alexandre [2] ; Prospero, Andre G. [1] ; Mello, Fabio P. F. [1] ; Soares, Guilherme [1] ; Di Stasi, Luiz C. [2] ; Miranda, Jose R. A. [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ, Biosci Inst, Dept Phys & Biophys, Lab Biomagnetism, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Biosci Inst, Dept Pharmacol, Lab Phytomed Pharmacol & Biotechnol, Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Life Sciences; v. 236, NOV 1 2019.
Web of Science Citations: 0
Abstract

Aims: Inflammatory bowel disease is a chronic relapsing inflammation that affects the gastrointestinal tract, causing changes in colonic motility. The evolution of these changes is not completely understood and possibly related to symptoms that appear in different degrees of the intestinal inflammation. Therefore, our aim is evaluate during 14 days of assessment aspects of colonic contractility using 2,4,6-trinitrobenzenesulfonic acid (TNBS) model of inflammation in rats and associate the inflammatory process with colonic motility. Methods: Contractility and inflammatory parameters were assessed in the same animal in six different moments: before intestinal inflammation induction, 2, 5, 8, 11, and 14 days after induction. The mechanical activity was determined by alternating current biosusceptometry (ACB) and subdivided into rhythmic propagating ripples (RPR) and rhythmic propulsive motor complexes (RPMC). We assessed inflammation by determining myeloperoxidase activity in feces. Results: Transient and permanent changes were observed in colonic motility as a function of the inflammatory process evaluated through myeloperoxidase activity. We identified two contraction profiles: RPR and RPMC. The microscopic analysis demonstrated a depth of damage caused by an injury that was associated with changes in motility. Conclusions: We implemented a robust and adequate (specific) signal processing to quantify two measured colonic frequency patterns. Thus, we performed a detailed temporal analysis of the consequences of TNBS-induced inflammation on colonic motility in rats. Our approach enables further long-term assessments in the same animal with different mechanisms and duration of injury, remission, treatments and their motor consequences. (AU)

FAPESP's process: 13/09095-4 - Electrical and mechanical study of colonic motility under influence of ulcerative colitis
Grantee:Marcos Felipe de Freitas Calabresi
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 15/14923-9 - Instrumentation and applications by biosusceptometry alternating current: nanoparticles distribution studies, motility and quality control of solid dosage forms
Grantee:José Ricardo de Arruda Miranda
Support Opportunities: Regular Research Grants