Electrical and mechanical study of colonic motility under influence of ulcerative colitis

• Grant number: 13/09095-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): August 01, 2013
• Effective date (End): February 28, 2017
• Field of knowledge: Biological Sciences - Biophysics - Biophysics of Processes and Systems
• Principal Investigator: José Ricardo de Arruda Miranda
• Grantee: Marcos Felipe de Freitas Calabresi
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 10/07639-9 - Gastrointestinal motility: clinical investigations, physiopathology and biopharmaceutical, AP.TEM

Abstract

Ulcerative colitis is an inflammatory bowel disease (IBD) with currently increasing incidence, especially in developed countries. With poorly understood pathogenesis, this disease has its main symptoms, such as diarrhea and abdominal pain associated with altered colonic motility. This project aims to apply, in an unprecedented way, a specific instrumentation to investigate colonic motility behavior in normal colons and under ulcerative colitis influence. To acquire electrical activity, will be a Electromyography system (EMG), simultaneously to a Alternating Current Biosusceptometry system (ACB), recording the mechanical activity and externally monitoring a magnetic tracer incorporated to a test meal. The studies to be developed, will be performed in human and animal models, collecting data regarding colonic contraction activity. In the animal model, we will use Wistar male rats with induced colitis by a trinitrobenzene sulfonic acid (TBNS) solution rectally administered. Clinical studies will be realizedfrom a control group (volunteers) and a patients group from the University Hospital of Botucatu (HCFMB) that have moderate ulcerative colitis.It is expected that the results demonstrate the electric and motor responses of this disease and its effects on colonic motility, as well as the characteristics and influences of inflammation in different stages of recovery. The results also provides a standardize a methodology for colon assessment, which is quite incipient to the present. The comparison of human and animal model will make possible the development of new treatments and therapies, in addition to a better understanding of the disease, exploring some aspects about the pathophysiology involving increased intestinal motility. Therefore it is aimedto evaluate electrical and mechanical colonic activity and colonic segmental transit in rats and in humans, to evaluate the colonic mechanical activity and segmental colonic transit in situations involving colitis.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CALABRESI, MARCOS F. F.; TANIMOTO, ALEXANDRE; PROSPERO, ANDRE G.; MELLO, FABIO P. F.; SOARES, GUILHERME; DI STASI, LUIZ C.; MIRANDA, JOSE R. A.. Changes in colonic contractility in response to inflammatory bowel disease: Long-term assessment in a model of TNBS-induced inflammation in rats. Life Sciences, v. 236, NOV 1 2019. (13/09095-4, 15/14923-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CALABRESI, Marcos Felipe de Freitas. Evolution of electric and mechanical profiles of colonic motility in an animal model of inflammatory bowel disease. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.