Molecular signatures associated with prostate cancer cell line (PC-3) exposure to inactivated Zika virus

Autor(es):Mostrar menos -

Delafiori, Jeany ^[1] ; Lima, Estela de Oliveira ^[2] ; Dabaja, Mohamed Ziad ^[1] ; Dias-Audibert, Flavia Luisa ^[1] ; de Oliveira, Diogo Noin ^[1] ; Rodrigues Melo, Carlos Fernando Odir ^[1] ; Morishita, Karen Noda ^[1] ; Sales, Geovana Manzan ^[1] ; Tasca Gois Ruiz, Ana Lucia ^{[3, 4]} ; da Silva, Gisele Goulart ^[3] ; Lancellotti, Marcelo ^[5] ; Catharino, Rodrigo Ramos ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Pharmaceut Sci, Innovare Biomarkers Lab, UNICAMP, Campinas, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Med Sch, Botucatu, SP - Brazil

^[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, UNICAMP, Piracicaba, SP - Brazil

^[4] Univ Estadual Campinas, Sch Pharmaceut Sci, UNICAMP, Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Sch Pharmaceut Sci, Lab Biotechnol, UNICAMP, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, OCT 25 2019.
• Citações Web of Science: 0

Resumo

The recent outbreak of Zika virus (ZIKV) infection associated with microcephaly cases has elicited much research on the mechanisms involved in ZIKV-host cell interactions. It has been described that Zika virus impairs cell growth, raising a hypothesis about its oncolytic potential against cancer cells. ZIKV tumor cell growth inhibition was later confirmed for glioblastoma. It was also demonstrated that an inactivated ZIKV prototype (ZVp) based on bacterial outer membrane vesicles has antiproliferative activity upon other cancer cell lines, such as PC-3 prostate cancer cell. This study aims at understanding the pathways that might be involved with the antiproliferative effect of Zika virus against prostate cancer cells. A metabolomic approach based on high-resolution mass spectrometry analysis led to the identification of 21 statistically relevant markers of PC-3 cells treated with ZVp. The markers were associated with metabolic alterations that trigger lipid remodeling, endoplasmic reticulum stress, inflammatory mediators, as well as disrupted porphyrin and folate metabolism. These findings highlight molecular signatures of ZVp-induced response that may be involved on cellular pathways triggered by its antiproliferative effect. To our knowledge, this is the first reported metabolomic assessment of ZIKV effect on prostate cancer cells, a promising topic for further research. (AU)

• Processo FAPESP: 13/07607-8 - CMPO - Centro Multidisciplinar de Pesquisa em Obesidade e Doenças Associadas
• Beneficiário: Licio Augusto Velloso
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 16/17066-2 - Metabolômica e lipidômica do Zika vírus, do mosquito ao paciente
• Beneficiário: Carlos Fernando Odir Rodrigues Melo
• Modalidade de apoio: Bolsas no Brasil - Doutorado