Analysis of metabolite profiles of Saccharomyces cerevisiae strains suitable for butanol production

Autor(es)

Azambuja, Sueellen P. H. ^[1] ; Teixeira, Gleidson S. ^[1] ; Andrietta, Maria G. S. ^[2] ; Torres-Mayanga, Paulo C. ^[3] ; Forster-Carneiro, Tania ^[3] ; Rosa, Carlos A. ^[4] ; Goldbeck, Rosana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Engn, Lab Bioproc & Metab Engn, BR-13083862 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Chem Biol & Agr Pluridisciplinary Res Ctr CPQBA, BR-13148218 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Sch Food Engn, Dept Food Engn, Lab Bioengn & Water & Waste Treatment, BR-13083862 Campinas, SP - Brazil

^[4] Univ Fed Minas Gerais, Dept Microbiol, BR-31270901 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEMS Microbiology Letters; v. 366, n. 13 JUL 2019.
• Citações Web of Science: 0

Resumo

Butanol has advantages over ethanol as a biofuel. Although butanol is naturally produced by some Clostridium species, clostridial fermentation has inherent characteristics that prevent its industrial application. Butanol-producing Saccharomyces cerevisiae strains may be a solution to this problem. The aim of this study was to evaluate the ability of wild-type and industrial Brazilian strains of S. cerevisiae to produce n-butanol using glycine as co-substrate and evaluate the relationship between the production of this alcohol and other metabolites in fermented broth. Of the 48 strains analyzed, 25 were able to produce n-butanol in a glycine-containing medium. Strains exhibited different profiles of n-butanol, isobutanol, ethanol, glycerol and acetic acid production. Some wild-type strains showed substantial n-butanol production capability, for instance UFMG-CM-Y267, which produced about 12.7mg/L of butanol. Although this concentration is low, it demonstrates that wild-type S. cerevisiae can synthesize butanol, suggesting that selection and genetic modification of this microorganism could yield promising results. The findings presented here may prove useful for future studies aimed at optimizing S. cerevisiae strains for butanol production. (AU)

• Processo FAPESP: 18/05999-0 - Waste2energy: integração das tecnologias supercrítica e digestão anaeróbia para gestão de águas e resíduos alimentares
• Beneficiário: Tânia Forster Carneiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
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• Beneficiário: Telma Teixeira Franco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
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• Beneficiário: Rubens Maciel Filho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/04602-3 - Desenvolvimento de linhagens de Saccharomyces cerevisiae através da expressão heteróloga de celulases e engenharia evolutiva visando a sacarificação e fermentação simultânea da biomassa lignocelulósica
• Beneficiário: Rosana Goldbeck Coelho
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
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• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular