Evaluation of Cytopathological Techniques for the Diagnosis of Canine Visceral Leishmaniosis with Lymph Node Samples

Autor(es):Mostrar menos -

Guerr, J. M. ^{[1, 2]} ; Fernandes, N. C. C. A. ^{[1, 2]} ; Ressio, R. A. ^[1] ; Magno, J. A. ^[1] ; Kimura, L. M. ^[1] ; Barbosa, J. E. de R. ^[1] ; Bertollo, D. M. B. ^[3] ; Taniguchi, H. H. ^[1] ; Hiramoto, R. M. ^[1] ; Motoie, G. ^[1] ; Tolezano, J. E. ^[1] ; Cogliati, B. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Coordenadoria Controle Doencas, Secretaria Estado Saude Sao Paulo, Inst Adolfo Lutz, Av Prof Orlando Marques de Paiva 87, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Patol, Fac Med Vet & Zootecnia, Av Prof Orlando Marques de Paiva 87, Sao Paulo - Brazil

^[3] Secretaria Estado Saude Sao Paulo, Coordenadoria Controle Doencas, Ctr Lab Reg Inst Adolfo Lutz Sao Jose do Rio Pret, Rua Alberto Sufredini Bertoni 2325, Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Comparative Pathology; v. 172, p. 62-71, OCT 2019.
• Citações Web of Science: 0

Resumo

The identification of the parasite in cytological smears of lymph node aspirates is a widely applied technique for the direct diagnosis of Leishmania spp. infection, especially in endemic areas. Although very specific, this method has limited sensitivity, and improving the technique would be highly desirable. This study aimed to evaluate the efficacy of conventional smear cytology (SC), liquid-based cytology (LBC), cell block (CB) stained with haematoxylin and eosin (HE) and immunocytochemistry (ICC), and formalin-fixed paraffin wax-embedded tissue immunohistochemistry (FFPE-IHC) compared with serology and polymerase chain reaction for the diagnosis of canine visceral leishmaniosis (CVL) in lymphoid tissue. The use of a preservative medium and centrifugation for cytological samples reduced the number of unsatisfactory artefacts/background. Moreover, LBC allowed excellent cellular preservation and the application of ancillary techniques, such as CB and ICC. SC was the most accurate morphological diagnostic method (45.0%). CB-ICC alone or associated with SC demonstrated significantly higher sensitivity (70.0% and 72.0%, respectively) when compared with SC alone (34.00%). CB-ICC was found to be more effective in the detection of infected animals with mild clinical signs, similar to FFPE-IHC. The specificity and positive predictive value were similar between all methods. Finally, the detection limit for CB-ICC and SC + CB-ICC was identical (18.46 amastigotes/ mm(2)). Our study suggests that CB-ICC is a promising tool for improvement of the cytopathological diagnosis of CVL and may be applied in routine epidemiological screening. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/51267-4 - Aprimoramento do diagnóstico etiológico das leishmanioses no estado de São Paulo: bases para a implantação de uma rede de laboratórios para a identificação das rotas de disseminação e monitoramento da diversidade fenotípica e genotípica dos protozoários do gênero Leishmania
• Beneficiário: José Eduardo Tolezano
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa em Políticas Públicas para o SUS