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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Red blood cells microparticles are associated with hemolysis markers and may contribute to clinical events among sickle cell disease patients

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Autor(es):
Olatunya, Oladele Simeon [1, 2] ; Lanaro, Carolina [1] ; Longhini, Ana Leda [1] ; Franco Penteado, Carla Fernanda [1] ; Fertrin, Kleber Y. [1] ; Adekile, Adekunle [3] ; Saad, Sara T. O. [1] ; Costa, Fernando Ferreira [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, UNICAMP, Hematol & Hemotherapy Ctr Hemoctr, Rua Carlos Chagas 480, BR-13083970 Campinas, SP - Brazil
[2] Ekiti State Univ, Coll Med, Dept Pediat, Ado Ekiti - Nigeria
[3] Kuwait Univ, Fac Med, Dept Pediat, Kuwait - Kuwait
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: ANNALS OF HEMATOLOGY; v. 98, n. 11, p. 2507-2521, NOV 2019.
Citações Web of Science: 0
Resumo

Microparticles are sub-micron vesicles possessing protein and other materials derived from the plasma membrane of their parent cells, and literature suggests that they may have a role in the pathophysiology and downstream manifestations of sickle cell disease (SCD). The contributions of red blood cells microparticles (RMP) to the pathogenic mechanisms and clinical phenotypes of SCD are largely unknown. There is a controversy as to whether the proportions of intravascular hemolysis (approximately <= 30% of total hemolysis) would be enough to explain some complications seen in patients with SCD. We investigated RMP among 138 SCD patients and 39 HbAA individuals. Plasma RMPs were quantified by flow cytometry, plasma hemoglobin and heme by colorimetric assays, and haptoglobin and hemopexin by ELISA. The patients had higher RMP, plasma hemoglobin, and heme compared to the controls. On the contrary, haptoglobin and hemopexin were depleted in the patients. The RMP correlated positively with heme, lactate dehydrogenase, plasma hemoglobin, serum bilirubin, reticulocyte counts, and tricuspid regurgitant jet velocity of the patients. Contrarily, it correlated negatively with HbF, hemopexin, red blood cells counts, hemoglobin concentration, and haptoglobin. Although patients treated with hydroxyurea had lower RMP, this did not attain statistical significance. Patients with sickle leg ulcer and elevated tricuspid regurgitant jet velocity had higher levels of RMP. In conclusion, these data suggest that RMPs are associated with hemolysis and may have important roles in the pathophysiology and downstream complications of SCD. (AU)

Processo FAPESP: 14/00984-3 - Doenças dos glóbulos vermelhos: fisiopatologia e novas abordagens terapêuticas
Beneficiário:Fernando Ferreira Costa
Linha de fomento: Auxílio à Pesquisa - Temático