Liquefied wheat bran as carbon source and inducer in high-solids submerged cultivation of Aspergillus niger for xylanase production

Autor(es)

Amorim, Cristiane C. ^[1] ; Farinas, Cristiane S. ^{[2, 3]} ; Miranda, Everson A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Chem Engn, Av Albert Einstein 500, BR-13083852 Campinas, SP - Brazil

^[2] Embrapa Instrumentat, Rua 15 Novembro 1452, BR-13561206 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Grad Program Chem Engn, Rodovia Washington Luis S-N, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY; v. 21, SEP 2019.
• Citações Web of Science: 0

Resumo

High-solids submerged cultivation for microbial production of industrially relevant enzymes can be facilitated by using a liquefied substrate as carbon source and inducer. Here, liquefied wheat bran (WB) was evaluated as carbon source and inducer in high-solids submerged cultivation (20%, w/w) of a filamentous fungus, A. niger, for xylanase production. A systematic study (one factor at a time) of the use of liquefied WB under different conditions of temperature, pH, enzyme loading, and reaction time was performed. This study revealed that xylanase production was favored by a substrate liquefied using the optimum pH (4.8) and temperature conditions (50 degrees C) of the commercial enzymatic preparation (2.8 FPU/g solids), while the liquefaction time was not significant above 6 h. The proposed strategy resulted in xylanase activity 5-fold higher than for a cultivation with non-liquefied substrate (control). These findings should contribute to the development of xylanase production processes using high solids loading submerged cultivation in conventional large-scale bioreactors. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 14/19000-3 - Simplificação do processo de conversão de biomassa a etanol por meio da integração da produção de enzimas in-house
• Beneficiário: Cristiane Sanchez Farinas
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular