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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Liquefied wheat bran as carbon source and inducer in high-solids submerged cultivation of Aspergillus niger for xylanase production

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Author(s):
Amorim, Cristiane C. [1] ; Farinas, Cristiane S. [2, 3] ; Miranda, Everson A. [1]
Total Authors: 3
Affiliation:
[1] Univ Estadual Campinas, Sch Chem Engn, Av Albert Einstein 500, BR-13083852 Campinas, SP - Brazil
[2] Embrapa Instrumentat, Rua 15 Novembro 1452, BR-13561206 Sao Carlos, SP - Brazil
[3] Univ Fed Sao Carlos, Grad Program Chem Engn, Rodovia Washington Luis S-N, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY; v. 21, SEP 2019.
Web of Science Citations: 0
Abstract

High-solids submerged cultivation for microbial production of industrially relevant enzymes can be facilitated by using a liquefied substrate as carbon source and inducer. Here, liquefied wheat bran (WB) was evaluated as carbon source and inducer in high-solids submerged cultivation (20%, w/w) of a filamentous fungus, A. niger, for xylanase production. A systematic study (one factor at a time) of the use of liquefied WB under different conditions of temperature, pH, enzyme loading, and reaction time was performed. This study revealed that xylanase production was favored by a substrate liquefied using the optimum pH (4.8) and temperature conditions (50 degrees C) of the commercial enzymatic preparation (2.8 FPU/g solids), while the liquefaction time was not significant above 6 h. The proposed strategy resulted in xylanase activity 5-fold higher than for a cultivation with non-liquefied substrate (control). These findings should contribute to the development of xylanase production processes using high solids loading submerged cultivation in conventional large-scale bioreactors. (AU)

FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 14/19000-3 - Simplification of the biomass to ethanol conversion process by integration with the production of enzymes in-house
Grantee:Cristiane Sanchez Farinas
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants