Pharmacokinetic disposition of erythraline in rats after intravenous administration

Autor(es)

Demarque, Daniel P. ^[1] ; Callejon, Daniel R. ^[1] ; Pinto, Larissa G. ^[1] ; Gouvea, Dayana R. ^[1] ; de Moraes, Natalia V. ^[2] ; Lopes, Joao L. C. ^[1] ; Lopes, Jose N. C. ^[3] ; Lopes, Norberto P. ^[1] ; Guaratini, Thais ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Fis & Quim, Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Principios Ativos Nat & Toxicol, Araraquara, SP - Brazil

^[3] Lychnoflora Pesquisa & Desenvolvimento Prod Nat L, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 29, n. 6, p. 773-777, NOV-DEC 2019.
• Citações Web of Science: 0

Resumo

Erythraline is the major alkaloid produced by Erythrina verna Vell., Fabaceae, a plant used in folk medicine and phytotherapeutic products to treat anxiety and sleep disorders. This study aimed to investigate the pharmacokinetic parameters of erythraline after intravenous administration in rats. For the analysis of erythraline in rat plasma, a method was developed and validated using UHPLC-MS/MS. Pharmacokinetic parameters were estimated by non-compartmental analysis. The metabolite 8-oxo-erythraline observed in previous biomimetic model studies was monitored during in vivo experiments. The quantification limit was 5 ng/ml within a linear range of 5-2000 ng/ml. After an intravenous dose of 1 mg/kg, the following pharmacokinetic parameters were observed: elimination half-life of 44.2 min; total clearance of 42.1 ml/min/kg and volume of distribution of 2085 ml/kg. In summary, a precise, accurate and selective UHPLC-MS/MS method was developed and successfully applied to investigate the pharmacokinetics of erythraline in rats. The metabolite 8-oxo-erythraline was observed in rat plasma after 20 min of erythraline administration. (C) 2019 Published by Elsevier Editora Ltda. on behalf of Sociedade Brasileira de Farmacognosia. (AU)

• Processo FAPESP: 14/50265-3 - Metabolismo e distribuição de xenobióticos naturais e sintéticos: da compreensão dos processos reacionais à geração de imagens teciduais
• Beneficiário: Norberto Peporine Lopes
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 14/18052-0 - Histolocalização de taninos e antraquinonas com atuação no sistema gastrointestinal
• Beneficiário: Daniel Pecoraro Demarque
• Linha de fomento: Bolsas no Brasil - Doutorado