Challenges in providing compatible blood with Rh genotype-matching in Brazilian patients with sickle cell disease

Autor(es)

Santos, T. D. ^[1] ; Macedo, M. D. ^[1] ; Menegati, S. F. P. ^[1] ; Gilli, S. ^[1] ; Castilho, L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Hemoctr Campinas, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Transfusion Medicine; OCT 2019.
• Citações Web of Science: 0

Resumo

Objectives The aim of this study was to verify the possibility of performing prophylactic Rh genotype-matching in Brazilian patients with sickle cell disease (SCD) and identify the genotypes that are lacking or insufficient in our donor cohort. Background Rh alloimmunisation is still a challenge in transfused patients with SCD. Rh genotype-matching may mitigate Rh alloimmunisation. Methods/Materials We examined the transfusion requests for antigen-matched donor units in SCD patients with Rh variants and compared the Rh altered alleles in the patients to the Rh allele frequency in a selected donor population. For each patient and donor, RBC antigen genotyping was performed using HEA, RHD and RHCE BeadChip arrays. Sequencing was used to clarify inconclusive results. Twenty-one patients and 956 Brazilian blood donors were genotyped. Results According to our matching strategies, 47 center dot 6% of patients filled most of their unit requests, but 52 center dot 4% of patients had insufficient donors to fill their annual transfusion needs. We found different combinations of RHCE variant alleles in patients and donors, but the most frequent genotypes that are lacking or insufficient in our donor cohort are those associated with the lack of hr(B) and hr(S) high prevalence antigens and those co-inherited with altered RHD alleles. Conclusion Our study shows that the provision of compatible blood with Rh genotype-matching in Brazilian patients with SCD can be feasible but challenging and, efficient strategies of recruitment of African-Brazilian donors must be developed. (AU)

• Processo FAPESP: 14/00984-3 - Doenças dos glóbulos vermelhos: fisiopatologia e novas abordagens terapêuticas
• Beneficiário: Fernando Ferreira Costa
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/07559-9 - Análise de polimorfismos genéticos associados com aloimunização eritrocitária e desenvolvimento de painéis de referência para genotipagem de variantes Rh: impacto na segurança transfusional de pacientes com doença falciforme
• Beneficiário: Lilian Maria de Castilho
• Modalidade de apoio: Auxílio à Pesquisa - Regular