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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Challenges in providing compatible blood with Rh genotype-matching in Brazilian patients with sickle cell disease

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Author(s):
Santos, T. D. [1] ; Macedo, M. D. [1] ; Menegati, S. F. P. [1] ; Gilli, S. [1] ; Castilho, L. [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Hemoctr Campinas, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Transfusion Medicine; OCT 2019.
Web of Science Citations: 0
Abstract

Objectives The aim of this study was to verify the possibility of performing prophylactic Rh genotype-matching in Brazilian patients with sickle cell disease (SCD) and identify the genotypes that are lacking or insufficient in our donor cohort. Background Rh alloimmunisation is still a challenge in transfused patients with SCD. Rh genotype-matching may mitigate Rh alloimmunisation. Methods/Materials We examined the transfusion requests for antigen-matched donor units in SCD patients with Rh variants and compared the Rh altered alleles in the patients to the Rh allele frequency in a selected donor population. For each patient and donor, RBC antigen genotyping was performed using HEA, RHD and RHCE BeadChip arrays. Sequencing was used to clarify inconclusive results. Twenty-one patients and 956 Brazilian blood donors were genotyped. Results According to our matching strategies, 47 center dot 6% of patients filled most of their unit requests, but 52 center dot 4% of patients had insufficient donors to fill their annual transfusion needs. We found different combinations of RHCE variant alleles in patients and donors, but the most frequent genotypes that are lacking or insufficient in our donor cohort are those associated with the lack of hr(B) and hr(S) high prevalence antigens and those co-inherited with altered RHD alleles. Conclusion Our study shows that the provision of compatible blood with Rh genotype-matching in Brazilian patients with SCD can be feasible but challenging and, efficient strategies of recruitment of African-Brazilian donors must be developed. (AU)

FAPESP's process: 14/00984-3 - Red blood cell disorders: pathophysiology and new therapeutic approaches
Grantee:Fernando Ferreira Costa
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/07559-9 - Analysis of genetic polymorphisms associated with red blood cell alloimmunization and development of reference panels for RH genotyping to improve transfusion outcomes in patients with sickle cell disease
Grantee:Lilian Maria de Castilho
Support type: Regular Research Grants