Improving the Yields and Reaction Rate in the Ethanolysis of Soybean Oil by Using Mixtures of Lipase CLEAs

Autor(es)

Ramos, Margarita Diaz ^[1] ; Miranda, Leticia Passos ^[1] ; Fernandez-Lafuente, Roberto ^[2] ; Kopp, William ^[3] ; Tardioli, Paulo Waldir ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Chem Engn, Postgrad Program Chem Engn, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] CSIC, ICP, Dept Biocatalisis, Campus UAM CSIC, E-28049 Madrid - Spain

^[3] Kopp Technol KTech, Rua Alfredo Lopes 1717, BR-13560190 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 24, n. 23 DEC 2019.
• Citações Web of Science: 1

Resumo

Due to the heterogeneity of oils, the use of mixtures of lipases with different activity for a large number of glycerol-linked carboxylic acids that compose the substrate has been proposed as a better alternative than the use of one specific lipase preparation in the enzymatic synthesis of biodiesel. In this work, mixtures of lipases from different sources were evaluated in their soluble form in the ethanolysis of soybean oil. A mixture of lipases (50% of each lipase, in activity basis) from porcine pancreas (PPL) and Thermomyces lanuginosus lipase (TLL) gave the highest fatty acid ethyl ester (FAEE) yield (around 20 wt.%), while the individual lipases gave FAEE yields 100 and 5 times lower, respectively. These lipases were immobilized individually by the cross-linked enzyme aggregates (CLEAs) technique, yielding biocatalysts with 89 and 119% of expressed activity, respectively. A mixture of these CLEAs (also 50% of each lipase, in activity basis) gave 90.4 wt.% FAEE yield, while using separately CLEAs of PPL and TLL, the FAEE yields were 84.7 and 75.6 wt.%, respectively, under the same reaction conditions. The mixture of CLEAs could be reused (five cycles of 6 h) in the ethanolysis of soybean oil in a vortex flow-type reactor yielding an FAEE yield higher than 80% of that of the first batch. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 11/23194-0 - Síntese enzimática de ésteres de ácidos graxos com aquecimento assistido por micro-ondas
• Beneficiário: Paulo Waldir Tardioli
• Modalidade de apoio: Auxílio à Pesquisa - Regular