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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Improving the Yields and Reaction Rate in the Ethanolysis of Soybean Oil by Using Mixtures of Lipase CLEAs

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Author(s):
Ramos, Margarita Diaz [1] ; Miranda, Leticia Passos [1] ; Fernandez-Lafuente, Roberto [2] ; Kopp, William [3] ; Tardioli, Paulo Waldir [1]
Total Authors: 5
Affiliation:
[1] Univ Fed Sao Carlos, Dept Chem Engn, Postgrad Program Chem Engn, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil
[2] CSIC, ICP, Dept Biocatalisis, Campus UAM CSIC, E-28049 Madrid - Spain
[3] Kopp Technol KTech, Rua Alfredo Lopes 1717, BR-13560190 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Molecules; v. 24, n. 23 DEC 2019.
Web of Science Citations: 1
Abstract

Due to the heterogeneity of oils, the use of mixtures of lipases with different activity for a large number of glycerol-linked carboxylic acids that compose the substrate has been proposed as a better alternative than the use of one specific lipase preparation in the enzymatic synthesis of biodiesel. In this work, mixtures of lipases from different sources were evaluated in their soluble form in the ethanolysis of soybean oil. A mixture of lipases (50% of each lipase, in activity basis) from porcine pancreas (PPL) and Thermomyces lanuginosus lipase (TLL) gave the highest fatty acid ethyl ester (FAEE) yield (around 20 wt.%), while the individual lipases gave FAEE yields 100 and 5 times lower, respectively. These lipases were immobilized individually by the cross-linked enzyme aggregates (CLEAs) technique, yielding biocatalysts with 89 and 119% of expressed activity, respectively. A mixture of these CLEAs (also 50% of each lipase, in activity basis) gave 90.4 wt.% FAEE yield, while using separately CLEAs of PPL and TLL, the FAEE yields were 84.7 and 75.6 wt.%, respectively, under the same reaction conditions. The mixture of CLEAs could be reused (five cycles of 6 h) in the ethanolysis of soybean oil in a vortex flow-type reactor yielding an FAEE yield higher than 80% of that of the first batch. (AU)

FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 11/23194-0 - Enzymatic synthesis of fatty esters using heating assisted by microwave
Grantee:Paulo Waldir Tardioli
Support Opportunities: Regular Research Grants