Marine Bacteria from Rocas Atoll as a Rich Source of Pharmacologically Active Compounds

Autor(es)

Velasco-Alzate, Karen Y. ^[1] ; Bauermeister, Anelize ^{[1, 2]} ; Tangerina, Marcelo M. P. ^[3] ; Lotufo, Tito M. C. ^[4] ; Ferreira, Marcelo J. P. ^[3] ; Jimenez, Paula C. ^[5] ; Padilla, Gabriel ^[6] ; Lopes, Norberto P. ^[2] ; Costa-Lotufo, Leticia V. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Farmacol, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS, Dept Fis & Quim, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Inst Oceanog, Dept Oceanog Biol, BR-05508120 Sao Paulo, SP - Brazil

^[5] Univ Fed Sao Paulo, Dept Ciencias Mar, BR-11015020 Santos, SP - Brazil

^[6] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MARINE DRUGS; v. 17, n. 12 DEC 2019.
• Citações Web of Science: 0

Resumo

Rocas Atoll is a unique environment in the equatorial Atlantic Ocean, hosting a large number of endemic species, however, studies on the chemical diversity emerging from this biota are rather scarce. Therefore, the present work aims to assess the metabolomic diversity and pharmacological potential of the microbiota from Rocas Atoll. A total of 76 bacteria were isolated and cultured in liquid culture media to obtain crude extracts. About one third (34%) of these extracts were recognized as cytotoxic against human colon adenocarcinoma HCT-116 cell line. 16S rRNA gene sequencing analyses revealed that the bacteria producing cytotoxic extracts were mainly from the Actinobacteria phylum, including Streptomyces, Salinispora, Nocardiopsis, and Brevibacterium genera, and in a smaller proportion from Firmicutes phylum (Bacillus). The search in the spectral library in GNPS (Global Natural Products Social Molecular Networking) unveiled a high chemodiversity being produced by these bacteria, including rifamycins, antimycins, desferrioxamines, ferrioxamines, surfactins, surugamides, staurosporines, and saliniketals, along with several unidentified compounds. Using an original approach, molecular networking successfully highlighted groups of compounds responsible for the cytotoxicity of crude extracts. Application of DEREPLICATOR+ (GNPS) allowed the annotation of macrolide novonestimycin derivatives as the cytotoxic compounds existing in the extracts produced by Streptomyces BRB-298 and BRB-302. Overall, these results highlighted the pharmacological potential of bacteria from this singular atoll. (AU)

• Processo FAPESP: 14/50265-3 - Metabolismo e distribuição de xenobióticos naturais e sintéticos: da compreensão dos processos reacionais à geração de imagens teciduais
• Beneficiário: Norberto Peporine Lopes
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 15/17177-6 - Abordagem integrada na prospecção sustentável de produtos naturais marinhos: da diversidade a substâncias anticâncer
• Beneficiário: Leticia Veras Costa Lotufo
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático