In vitro bioactivity approach of unripe genipap (Genipa americana L., Rubiaceae) fruit extract and its solid lipid microparticle

Autor(es)

Neri-Numa, Iramaia Angelica ^[1] ; DellaTorre, Adriana ^[2] ; Oriani, Vivian Boesso ^[1] ; Franch, Jr., Gilberto Carlos ^[3] ; Figueiredo Angolini, Celio Fernando ^[4] ; Hubinger, Miriam Dupas ^[1] ; Tasca Gois Ruiz, Ana Lucia ^[2] ; Pastore, Glaucia Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Food Engn, Campinas - Brazil

^[2] Univ Estadual Campinas, Fac Pharmaceut Sci, Campinas - Brazil

^[3] Univ Estadual Campinas, Integrated Ctr Childhood Onco Hematol Invest, Campinas - Brazil

^[4] Fed Univ ABC, Ctr Nat & Human Sci, Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 127, JAN 2020.
• Citações Web of Science: 0

Resumo

Growing awareness in favor of innovative and healthier alternatives is creating a noticeable shift from synthetic colorants to natural additives. And, such a swing in the consumer market is growing slowly but noticeably. In this context, genipap (Genipa americana L.) fruit represents an emerging source of blue colorants in Latin America with extensive application possibilities. This is despite the fact that there are few studies concerning its toxicity predictive factors. In this early-stage study we propose to investigate safety issues around genipap extract (IBBP); we also attempt to identify fingerprint profiling of both IBBP extract and solid lipid microparticles containing IBBP extract (SLM-IBBP) using in vitro assays. The main compounds identified were genipin, and genipin 1-beta-gentiobioside. Results indicated that IBBP extract, at 25 mu g/mL, was able to promote DNA damage in CHO-K1 cells, suggesting a genotoxic effect. On the other hand, the SLM-IBBP inhibited almost all cancer cell lines with GI(50) ranging from 0.25 mu g/mL to 43.5 mu g/mL. Also, IBBP-SLM seems to exert a desirable apoptosis induction (at 25 mu g/mL dosage). The next steps for our work, therefore, will focus on other nanoparticle formulation approaches, in particular with the use of natural Brazilian starch. An evaluation of the metabolism and distribution of microparticles, and their safety for food and pharmaceutical purposes, are also required. (AU)

• Processo FAPESP: 15/50333-1 - Cobra: nova plataforma de descoberta de compostos para identificação rápida e de baixo custo de bioativo com efeitos benéficos originários de plantas brasileiras
• Beneficiário: Glaucia Maria Pastore
• Modalidade de apoio: Auxílio à Pesquisa - Temático