| Texto completo | |
| Autor(es): |
Avila, Patricia F.
[1]
;
Franco Cairo, Joao Paulo L.
[2]
;
Damasio, Andre
[2]
;
Forte, Marcus B. S.
[1]
;
Goldbeck, Rosana
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Campinas UNICAMP, Dept Food Egineering, Sch Food Engn, Bioproc & Metab Engn Lab, Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Food Research International; v. 128, FEB 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Xylooligosaccharides (XOS) are non-digestible food ingredients with prebiotic properties for selectively promoting the growth of probiotics, which provide many health benefits and several applications in the food and pharmaceutical industry. The objective of this study was to optimize the concentration of commercial hemicellulases for the production of XOS, with a 2-6 polymerization degree, using a mixture of sugarcane bagasse and straw pretreated with ionic liquid or diluted sulfuric acid. The concentrations of enzymes endo-1,4-xylanase (NS50030, Novozyme (R)) and alpha-L-arabinofuranosidase (GH51) (Megazyme (R)) were optimized using a central composite rotatable design (CCRD). The xylooligosaccharides (XOS) released by hydrolysis were analyzed via capillary electrophoresis and quantified with HPAEC-PAD. The XOS profile obtained from the hydrolisis of the pretreated sugarcane biomass mixture (MPSA) was similar to that obtained with the hydrolisis of MBX, which provided higher xylobiose (X2) concentration. Our results also demonstrated that pretreatment with an ionic liquid favored the requirement of lower enzyme concentration in enzymatic hydrolysis for having provided a biomass with lower lignin content than the pretreatment with dilute sulfuric acid. It required up to 20% less of the optimum concentration of the endo-1,4-xylanase mixture to achieve similar values to those obtained with the biomass pretreated with dilute sulfuric acid, representing a possible alternative to reduce enzymatic cost. (AU) | |
| Processo FAPESP: | 15/20630-4 - Desenvolvimento integrado de biorrefinaria e planta de bioetanol de cana-de-açúcar com emissão zero de CO2: rotas para converter recursos renováveis em bioprodutos e bioeletricidade |
| Beneficiário: | Rubens Maciel Filho |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks |
| Beneficiário: | Telma Teixeira Franco |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 17/22669-0 - N-glicosilação e secreção de enzimas em fungos filamentosos |
| Beneficiário: | André Ricardo de Lima Damasio |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Regular |
| Processo FAPESP: | 16/04602-3 - Desenvolvimento de linhagens de Saccharomyces cerevisiae através da expressão heteróloga de celulases e engenharia evolutiva visando a sacarificação e fermentação simultânea da biomassa lignocelulósica |
| Beneficiário: | Rosana Goldbeck Coelho |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Regular |