Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Xylooligosaccharides production from a sugarcane biomass mixture: Effects of commercial enzyme combinations on bagasse/straw hydrolysis pretreated using different strategies

Full text
Author(s):
Avila, Patricia F. [1] ; Franco Cairo, Joao Paulo L. [2] ; Damasio, Andre [2] ; Forte, Marcus B. S. [1] ; Goldbeck, Rosana [1]
Total Authors: 5
Affiliation:
[1] Univ Campinas UNICAMP, Dept Food Egineering, Sch Food Engn, Bioproc & Metab Engn Lab, Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Food Research International; v. 128, FEB 2020.
Web of Science Citations: 0
Abstract

Xylooligosaccharides (XOS) are non-digestible food ingredients with prebiotic properties for selectively promoting the growth of probiotics, which provide many health benefits and several applications in the food and pharmaceutical industry. The objective of this study was to optimize the concentration of commercial hemicellulases for the production of XOS, with a 2-6 polymerization degree, using a mixture of sugarcane bagasse and straw pretreated with ionic liquid or diluted sulfuric acid. The concentrations of enzymes endo-1,4-xylanase (NS50030, Novozyme (R)) and alpha-L-arabinofuranosidase (GH51) (Megazyme (R)) were optimized using a central composite rotatable design (CCRD). The xylooligosaccharides (XOS) released by hydrolysis were analyzed via capillary electrophoresis and quantified with HPAEC-PAD. The XOS profile obtained from the hydrolisis of the pretreated sugarcane biomass mixture (MPSA) was similar to that obtained with the hydrolisis of MBX, which provided higher xylobiose (X2) concentration. Our results also demonstrated that pretreatment with an ionic liquid favored the requirement of lower enzyme concentration in enzymatic hydrolysis for having provided a biomass with lower lignin content than the pretreatment with dilute sulfuric acid. It required up to 20% less of the optimum concentration of the endo-1,4-xylanase mixture to achieve similar values to those obtained with the biomass pretreated with dilute sulfuric acid, representing a possible alternative to reduce enzymatic cost. (AU)

FAPESP's process: 15/20630-4 - Biorefinery development integrated to a bioethanol sugar cane plant with zero CO2 emission: routes to convert renewable resources to bio-products and bio-electricity
Grantee:Rubens Maciel Filho
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks
Grantee:Telma Teixeira Franco
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/22669-0 - N-glycosylation and enzymes secretion in filamentous fungi
Grantee:André Ricardo de Lima Damasio
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 16/04602-3 - Development of Saccharomyces cerevisiae strains through heterologous expression of cellulases and evolutionary engineering aiming for simultaneous saccharification and fermentation of lignocellulosic biomass
Grantee:Rosana Goldbeck Coelho
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants