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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Xylooligosaccharides production from a sugarcane biomass mixture: Effects of commercial enzyme combinations on bagasse/straw hydrolysis pretreated using different strategies

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Autor(es):
Avila, Patricia F. [1] ; Franco Cairo, Joao Paulo L. [2] ; Damasio, Andre [2] ; Forte, Marcus B. S. [1] ; Goldbeck, Rosana [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Campinas UNICAMP, Dept Food Egineering, Sch Food Engn, Bioproc & Metab Engn Lab, Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Food Research International; v. 128, FEB 2020.
Citações Web of Science: 0
Resumo

Xylooligosaccharides (XOS) are non-digestible food ingredients with prebiotic properties for selectively promoting the growth of probiotics, which provide many health benefits and several applications in the food and pharmaceutical industry. The objective of this study was to optimize the concentration of commercial hemicellulases for the production of XOS, with a 2-6 polymerization degree, using a mixture of sugarcane bagasse and straw pretreated with ionic liquid or diluted sulfuric acid. The concentrations of enzymes endo-1,4-xylanase (NS50030, Novozyme (R)) and alpha-L-arabinofuranosidase (GH51) (Megazyme (R)) were optimized using a central composite rotatable design (CCRD). The xylooligosaccharides (XOS) released by hydrolysis were analyzed via capillary electrophoresis and quantified with HPAEC-PAD. The XOS profile obtained from the hydrolisis of the pretreated sugarcane biomass mixture (MPSA) was similar to that obtained with the hydrolisis of MBX, which provided higher xylobiose (X2) concentration. Our results also demonstrated that pretreatment with an ionic liquid favored the requirement of lower enzyme concentration in enzymatic hydrolysis for having provided a biomass with lower lignin content than the pretreatment with dilute sulfuric acid. It required up to 20% less of the optimum concentration of the endo-1,4-xylanase mixture to achieve similar values to those obtained with the biomass pretreated with dilute sulfuric acid, representing a possible alternative to reduce enzymatic cost. (AU)

Processo FAPESP: 15/20630-4 - Desenvolvimento integrado de biorrefinaria e planta de bioetanol de cana-de-açúcar com emissão zero de CO2: rotas para converter recursos renováveis em bioprodutos e bioeletricidade
Beneficiário:Rubens Maciel Filho
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks
Beneficiário:Telma Teixeira Franco
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 17/22669-0 - N-glicosilação e secreção de enzimas em fungos filamentosos
Beneficiário:André Ricardo de Lima Damasio
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular
Processo FAPESP: 16/04602-3 - Desenvolvimento de linhagens de Saccharomyces cerevisiae através da expressão heteróloga de celulases e engenharia evolutiva visando a sacarificação e fermentação simultânea da biomassa lignocelulósica
Beneficiário:Rosana Goldbeck
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular