A high-carbohydrate diet induces greater inflammation than a high-fat diet in mouse skeletal muscle

Autor(es)

M.M. Antunes ; G. Godoy ; C.B. de Almeida-Souza ; B.A. da Rocha ; L.G. da Silva-Santi ; L.N. Masi ; F. Carbonera ; J.V. Visentainer ; R. Curi ; R.B. Bazotte

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 53, n. 3, p. -, 2020.
• Citações Web of Science: 0

Resumo

We previously reported that both the high-carbohydrate diet (HCD) and high-fat diet (HFD) given for two months promote lipid deposition and inflammation in the liver and brain of mice. The results obtained indicate a tissue-specific response to both diets. Herein, we compared the effects of HCD and HFD on fatty acid (FA) composition and inflammation in the gastrocnemius muscle. Male Swiss mice were fed with HCD or HFD for 1 or 2 months. Saturated FA (SFA), monounsaturated FA (MUFA), n-3 polyunsaturated FA (n-3 PUFA), and n-6 PUFA were quantified. The activities of stearoyl-CoA desaturase 1 (SCD-1), Δ-6 desaturase (D6D), elongase 6, and de novo lipogenesis (DNL) were estimated. As for indicators of the inflammatory tissue state, we measured myeloperoxidase (MPO) activity and gene expression of F4/80, tumor necrosis factor-α (TNF-α), interleukin (IL)-4, IL-6, and IL-10. The HCD led to a lower deposition of SFA, MUFA, n-3 PUFA, and n-6 PUFA compared to HFD. However, the HCD increased arachidonic acid levels, SFA/n-3 PUFA ratio, DNL, SCD-1, D6D, and MPO activities, and expression of IL-6, contrasting with the general idea that increased lipid deposition is associated with more intense inflammation. The HCD was more potent to induce skeletal muscle inflammation than the HFD, regardless of the lower lipid accumulation. (AU)

• Processo FAPESP: 18/09868-7 - Mecanismos celulares e moleculares envolvidos na resistência à insulina e inflamação em ratos Wistar obesos e Goto-Kakizaki magros: causas e associações com dieta e exercício físico
• Beneficiário: Rui Curi
• Linha de fomento: Auxílio à Pesquisa - Temático