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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A high-carbohydrate diet induces greater inflammation than a high-fat diet in mouse skeletal muscle

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Author(s):
M.M. Antunes [1] ; G. Godoy [2] ; C.B. de Almeida-Souza [3] ; B.A. da Rocha [4] ; L.G. da Silva-Santi [5] ; L.N. Masi [6] ; F. Carbonera [7] ; J.V. Visentainer [8] ; R. Curi [9] ; R.B. Bazotte [10]
Total Authors: 10
Affiliation:
[1] Universidade Estadual de Maringá. Departamento de Farmacologia e Terapêutica - Brasil
[2] Universidade Estadual de Maringá. Departamento de Farmacologia e Terapêutica - Brasil
[3] Universidade Estadual de Maringá. Departamento de Farmacologia e Terapêutica - Brasil
[4] Universidade Estadual de Maringá. Departamento de Farmacologia e Terapêutica - Brasil
[5] Universidade Estadual de Maringá. Departamento de Farmacologia e Terapêutica - Brasil
[6] Universidade Cruzeiro do Sul. Programa de Pós-Graduação Interdisciplinar em Ciências da Saúde - Brasil
[7] Universidade Estadual de Maringá. Departmento de Química - Brasil
[8] Universidade Estadual de Maringá. Departmento de Química - Brasil
[9] Universidade Cruzeiro do Sul. Programa de Pós-Graduação Interdisciplinar em Ciências da Saúde - Brasil
[10] Universidade Estadual de Maringá. Departamento de Farmacologia e Terapêutica - Brasil
Total Affiliations: 10
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 53, n. 3 2020-02-14.
Abstract

We previously reported that both the high-carbohydrate diet (HCD) and high-fat diet (HFD) given for two months promote lipid deposition and inflammation in the liver and brain of mice. The results obtained indicate a tissue-specific response to both diets. Herein, we compared the effects of HCD and HFD on fatty acid (FA) composition and inflammation in the gastrocnemius muscle. Male Swiss mice were fed with HCD or HFD for 1 or 2 months. Saturated FA (SFA), monounsaturated FA (MUFA), n-3 polyunsaturated FA (n-3 PUFA), and n-6 PUFA were quantified. The activities of stearoyl-CoA desaturase 1 (SCD-1), Δ-6 desaturase (D6D), elongase 6, and de novo lipogenesis (DNL) were estimated. As for indicators of the inflammatory tissue state, we measured myeloperoxidase (MPO) activity and gene expression of F4/80, tumor necrosis factor-α (TNF-α), interleukin (IL)-4, IL-6, and IL-10. The HCD led to a lower deposition of SFA, MUFA, n-3 PUFA, and n-6 PUFA compared to HFD. However, the HCD increased arachidonic acid levels, SFA/n-3 PUFA ratio, DNL, SCD-1, D6D, and MPO activities, and expression of IL-6, contrasting with the general idea that increased lipid deposition is associated with more intense inflammation. The HCD was more potent to induce skeletal muscle inflammation than the HFD, regardless of the lower lipid accumulation. (AU)

FAPESP's process: 18/09868-7 - Cellular and molecular mechanisms of insulin resistance and inflammation in obese Wistar rats and lean Goto-Kakizaki rats: causes and associations with diet and physical exercise
Grantee:Rui Curi
Support Opportunities: Research Projects - Thematic Grants