| Texto completo | |
| Autor(es): |
Bonfim-Silva, Ricardo
[1]
;
Salomao, Karina Bezerra
[1]
;
Costa de Andrade Pimentel, Thais Valeria
[2]
;
Branquinho de Oliveira Menezes, Camila Cristina
[3]
;
Bonini Palma, Patricia Vianna
[3]
;
Fontes, Aparecida Maria
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Genet, Ribeirao Preto Med Sch, Ave Bandeirantes, 3900 Monte Alegre, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Clin Med, Ribeirao Preto Med Sch, Ave Bandeirantes, 3900 Monte Alegre, BR-14049900 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Blood Ctr, Clin Hosp, Ribeirao Preto Med Sch, Ave Tenente Catao Roxo, 2501 Monte Alegre, BR-14049900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Cytotechnology; v. 71, n. 5, p. 893-903, OCT 2019. |
| Citações Web of Science: | 2 |
| Resumo | |
Medulloblastoma (MB) is the most common malignant brain tumor in children. Recent advances in molecular technologies allowed to classify MB in 4 major molecular subgroups: WNT, SHH, Group 3 and Group 4. In cancer research, cancer cell lines are important for examining and manipulating molecular and cellular process. However, it is important to know the characteristics of each cancer cell line prior to use, because there are some differences among them, even if they originate from the same cancer type. This study aimed to evaluate the similarities and differences among four human medulloblastoma cell lines, UW402, UW473, DAOY and ONS-76. The medulloblastoma cell lines were analyzed for (1) cell morphology, (2) immunophenotyping by flow cytometry for some specifics surface proteins, (3) expression level of adhesion molecules by RT-qPCR, (4) proliferative potential, (5) cell migration, and (6) in vivo tumorigenic potential. It was observed a relationship between cell growth and CDH1 (E-chaderin) adhesion molecule expression and all MB cell lines showed higher levels of CDH2 (N-chaderin) when compared to other adhesion molecule. ONS-76 showed higher gene expression of CDH5 (VE-chaderin) and higher percentage of CD144/VE-chaderin positive cells when compared to other MB cell lines. All MB cell lines showed low percentage of CD34, CD45, CD31, CD133 positive cells and high percentage of CD44, CD105, CD106 and CD29 positive cells. The DAOY cell line showed the highest migration potential, the ONS-76 cell line showed the highest proliferative potential and only DAOY and ONS-76 cell lines showed tumorigenic potential in vivo. MB cell lines showed functional and molecular differences among them, which it should be considered by the researchers in choosing the most suitable cellular model according to the study proposal. (AU) | |
| Processo FAPESP: | 11/20829-4 - Mecanismos epigenéticos de modulação dos genes HOX em meduloblastoma |
| Beneficiário: | Aparecida Maria Fontes |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/18664-7 - O papel do lncRNA Evf-2 na modulação dos genes Hox e potencial metastático de Meduloblastoma |
| Beneficiário: | Ricardo Bonfim Silva |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |