Effect of thyroid hormones on rat exocrine pancreas morphology and function

Autor(es)

Goulart-Silva, Francemilson ^[1] ; Marcal Pessoa, Ana Flavia ^[2] ; Figueredo Costa, Raquel Galvao ^[2] ; Bargi-Souza, Paula ^[3] ; Santos, Marinilce Fagundes ^[2] ; Nunes, Maria Tereza ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Ave Prof Dr Lineu Presses 1524, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo - Brazil

^[3] Univ Fed Minas Gerais, Inst Biol Sci, Dept Physiol & Biophys, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 245, MAR 15 2020.
• Citações Web of Science: 0

Resumo

Aim: The influence of thyroid hormones on exocrine pancreas function is poorly understood, and limited to the postnatal development period. Here, we evaluated the effects of hypo- and hyperthyroidism on the morphology and enzyme content of this tissue. Main methods: To induce hypothyroidism male Wistar rats were subjected to a thyroidectomy (Tx) or sham operated (SO). After 40 days, some of the Tx and SO rats were treated with T3 for 7 days. Following euthanization, the pancreas was removed and evaluated for morphology, as well as amylase, lipase and trypsin content, using histological and immunoreactive techniques analyses, respectively. Serum amylase levels were also evaluated. Key findings: The pancreatic acinar cells of Tx rats were smaller, exhibited reduced Haematoxyllin stained areas, and contained lower amylase and lipase levels, indicative of low cell activity. Tx rats also presented higher collagen levels, and high trypsin content in pancreatic extracts. Interestingly, T3 administration reversed the observed acinar cell alterations and restored pancreatic enzyme content, by augmenting amylase and lipase and attenuating trypsin levels, but failed to change collagen content. Increased levels of lipase and decreased trypsin were also observed in T3-treated SO rats. Significance: Thyroid hormones play an important role in acinar cell morphology and function. In the hypothyroid state there is a decrease in pancreatic enzyme levels that is restored with T3 treatment. In addition to participating in insulin sensitivity and glycemic control, THs also modulate enzyme expression and activity in the exocrine pancreas, consequently, delivering metabolic substrates to specific organs and tissues. (AU)

• Processo FAPESP: 08/56446-9 - Analise molecular das acoes pos-transcricionais dos hormonios tiroideanos nas celulas beta pancreaticas e suas repercussoes sobre a sintese e secrecao de insulina
• Beneficiário: Francemilson Goulart da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado