Adequate Placental Sampling for the Diagnosis and Characterization of Placental Infection by Zika Virus

Autor(es):Mostrar menos -

Venceslau, Emanuella Meneses ^[1] ; Siqueira Guida, Jose Paulo ^[1] ; Nobrega, Guilherme de Moraes ^[2] ; Samogim, Ana Paula ^[2] ; Parise, Pierina Lorencini ^[2] ; Japecanga, Rodolfo Rosa ^[1] ; de Toledo-Teixeira, Daniel Augusto ^[2] ; Forato, Julia ^[2] ; Antolini-Tavares, Arthur ^[3] ; Souza, Arethusa ^[3] ; Altemani, Albina ^[3] ; Consonni, Silvio Roberto ^[4] ; Passini, Renato ^[1] ; Amaral, Eliana ^[1] ; Proenca-Modena, Jose Luiz ^[2] ; Costa, Maria Laura ^[1] ; Network, Zika-Unicamp

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Med Sci, Dept Obstet & Gynecol, Campinas - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Campinas - Brazil

^[3] Univ Estadual Campinas, Sch Med Sci, Dept Pathol Anat, Campinas - Brazil

^[4] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 11, FEB 21 2020.
• Citações Web of Science: 0

Resumo

The detection of Zika virus (ZIKV) in immunoprivileged anatomical sites, potential sites for viral persistence, may guide the confirmation of undefined cases of ZIKV infection and also bring to light unknown pathways of viral transmission. Thus, this study aimed to characterize ZIKV infection in stratified, standardized placental samples in women with exanthematic febrile manifestations during pregnancy and compare findings to the standard investigation protocol of official health agencies. To this end, a case series of placental findings within a prospective cohort study was conducted over a period of 24 months. Serum/urine were obtained at the time of clinical case identification. Placental sampling was performed following standard investigation protocol (samples of 1.0 cm sent to a reference laboratory) and in a systematic way at various regions, such as chorionic plate, chorionic villi, basal plate, amniotic membrane, and umbilical cord, for subsequent ZIKV identification and quantification. Clinical information was obtained and histological preparation with hematoxylin-eosin staining for morphological evaluation was performed. This case series included 17 placentas systematically collected. Of these, 14 were positive by qRT-PCR for ZIKV, 5 in the umbilical cord, 7 in the amniotic membrane, 7 in the chorionic plate, 13 in the chorionic villi, and 7 in the basal plate, whereas none were reported by the reference laboratory. The most common morphological and anatomopathological findings were increased stromal cellularity, villitis, calcification, maternal vascular malperfusion, placental hypoplasia, and maternal-fetal hemorrhage (intervillous thrombi). Seven women presented positive testing for ZIKV in serological and/or molecular tests during gestation in urine. While viral quantification in urine ranged from 10(1) to 10(3) FFU eq/ml, that in different placental regions ranged from 10(3) to 10(8) FFU eq/g. Thus, ZIKV can infect different regions of the placenta and umbilical cord of pregnant women, showing that the systematic collection and adequate storage of the placenta is fundamental for the detection of ZIKV in this organ. The detection of ZIKV in the placenta after several months of initial symptoms suggests that this tissue may be a site for viral persistence during pregnancy. (AU)

• Processo FAPESP: 17/26908-0 - O papel de IRF-5 na patogênese e neurovirulência de Oropouche vírus
• Beneficiário: Pierina Lorencini Parise
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 16/00194-8 - Patogênese e neurovirulência de vírus emergentes no Brasil
• Beneficiário: José Luiz Proença Módena
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/02402-0 - O papel da resposta imune adaptativa na neuroinvasão por Oropouche e Zika vírus
• Beneficiário: Daniel Augusto de Toledo Teixeira
• Modalidade de apoio: Bolsas no Brasil - Mestrado