The Leptospira interrogans LIC10774 is a multifunctional surface protein that binds calcium and interacts with host components

Autor(es)

Passalia, Felipe Jose ^{[1, 2]} ; Carvalho, Eneas ^[3] ; Heinemann, Marcos Bryan ^[4] ; Vieira, Monica Larucci ^[5] ; Nascimento, Ana Lucia T. O. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Desenvolvimento Vacinas, BR-05503900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Ciencias Biomed, Programa Posgrad Interunidades Biotecnol, BR-05508900 Sao Paulo - Brazil

^[3] Inst Butantan, Lab Bacteriol, BR-05503900 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med Vet & Zootecnia, Lab Zoonoses Bacterianas, Sao Paulo - Brazil

^[5] Univ Fed Minas Gerais UFMG, ICB, Dept Microbiol, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROBIOLOGICAL RESEARCH; v. 235, MAY 2020.
• Citações Web of Science: 0

Resumo

Leptospirosis is a global re-emerging zoonosis, caused by pathogenic bacteria of the genus Leptospira. Humans are infected mainly through contact with contaminated water or soil. The understanding of the molecular mechanisms of leptospirosis through the characterization of unknown outer membrane proteins may contribute to the development of new treatments, diagnostic methods and vaccines. We have identified using bioinformatics analysis a protein that is encoded by the gene LIC10774, predicted to be localized at the leptospiral outer membrane and exhibit beta-roll folding. Surface exposure was confirmed by flow cytometry, ELISA and immunofluorescence-based confocal microscopy. Through circular dichroism spectroscopy and hydrophobic dye binding we have shown that rLIC10774 binds calcium ions, which imposes changes to secondary and tertiary structures. The recombinant protein was capable of binding to several host extracellular matrix and serum components. Therefore, we describe LIC10774 as a calcium-binding protein exposed in the outer surface of pathogenic leptospires with possible multifunctional roles in adhesion to host tissues, evasion of the immune system and participation in dissemination processes during leptospirosis. In addition, we hypothesize that the calcium binding is important for temperature-dependent functional roles during leptospirosis. (AU)

• Processo FAPESP: 17/00236-5 - As plaquetas na interface da modulação da imunidade, inflamação e hemostasia na leptospirose
• Beneficiário: Mônica Larucci Vieira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 14/50981-0 - Busca de proteínas de superfície nas sequências do genoma da Leptospira interrogans: caracterização funcional e imunológica para o entendimento de mecanismos envolvidos na patogênese de bactéria
• Beneficiário: Ana Lucia Tabet Oller do Nascimento
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/01102-2 - Caracterização bioquímica e imunológica de proteínas hipotéticas de superfície de Leptospira interrogans
• Beneficiário: Felipe José Passalia
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 18/07054-2 - As plaquetas na interface da modulação da imunidade, inflamação e hemostasia na leptospirose
• Beneficiário: Mônica Larucci Vieira
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores