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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Interplay between two quorum sensing-regulated pathways, violacein biosynthesis and VacJ/Yrb, dictates outer membrane vesicle biogenesis in Chromobacterium violaceum

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Autor(es):
Batista, Juliana H. [1] ; Leal, Fernanda C. [1] ; Fukuda, Taise T. H. [2] ; Alcoforado Diniz, Juliana [1] ; Almeida, Fausto [3] ; Pupo, Monica T. [2] ; da Silva Neto, Jose F. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol Bioagentes Patogen, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: ENVIRONMENTAL MICROBIOLOGY; v. 22, n. 6 MAY 2020.
Citações Web of Science: 0
Resumo

Outer membrane vesicles (OMVs) are lipid nanoparticles released by Gram-negative bacteria, which play multiple roles in bacterial physiology and adaptation to diverse environments. In this work, we demonstrate that OMVs released by the environmental pathogen Chromobacterium violaceum deliver the antimicrobial compound violacein to competitor bacteria, mediating its toxicity in vivo at a long distance. OMVs purified by ultracentrifugation from the wild-type strain, but not from a violacein-abrogated mutant Delta vioABCDE, contained violacein and inhibited several Gram-positive bacteria. Competition tests using co-culture and transwell assays indicated that the C. violaceum wild-type strain killed Staphylococcus aureus better than the Delta vioABCDE mutant strain. We found that C. violaceum achieves growth phase-dependent OMV release by the concerted expression of two quorum sensing (QS)-regulated pathways, namely violacein biosynthesis and VacJ/Yrb system. Although both pathways were activated at high cell density in a QS-dependent manner, the effect on vesiculation was the opposite. While the Delta vioABCDE mutant produced twofold fewer vesicles than the wild-type strain, indicating that violacein induces OMV biogenesis for its own delivery, the Delta vacJ and Delta yrbE mutants were hypervesiculating strains. Our findings uncovered QS-regulated pathways involved in OMV biogenesis used by C. violaceum to package violacein into OMVs for interbacterial competition. (AU)

Processo FAPESP: 18/01388-6 - Homeostase de ferro em Chromobacterium violaceum: mecanismos regulatórios, sistemas de captação e papel em virulência
Beneficiário:José Freire da Silva Neto
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/25445-9 - Bases genéticas da formação de biofilme em Chromobacterium violaceum
Beneficiário:Juliana Alcoforado Diniz
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/50954-0 - Novos agentes terapêuticos obtidos de bactérias simbiontes de invertebrados brasileiros
Beneficiário:Mônica Tallarico Pupo
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
Processo FAPESP: 15/26349-5 - Estudo químico e ecológico de microrganismos associados à simbiose Cecropia-Azteca
Beneficiário:Taise Tomie Hebihara Fukuda
Linha de fomento: Bolsas no Brasil - Doutorado Direto