Analysis of SIRT1 Expression in Plasma and in an In Vitro Model of Preeclampsia

Autor(es)

Viana-Mattioli, Sarah ^[1] ; Nunes, Priscila ^[2] ; Cavalli, Ricardo ^[3] ; Sandrim, Valeria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Inst Biosci Botucatu, Dept Pharmacol, BR-18680000 Botucatu, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Med Sch, BR-18680000 Botucatu, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Gynecol & Obstet, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY; v. 2020, APR 28 2020.
• Citações Web of Science: 0

Resumo

Preeclampsia (PE) is a pregnancy-specific disorder that affects 3-8% expecting mothers worldwide being one of the main causes of maternal and fetal morbidity and mortality. The search for altered circulating molecules in PE is an important target to better understand the pathophysiology of this disease. Therefore, we evaluated Sirtuin-1 (SIRT1) concentration in plasma from healthy pregnant (HP) women, gestational hypertensive women (GH), and preeclampsia women (PE) via enzyme-linked immunosorbent assay (ELISA). We also measured intracellular SIRT1 in HUVECs incubated with plasma from PE patients compared to HP and GH via Western Blot Assay. Statistical differences were considered when p<0.05. SIRT1 was downregulated in PE compared to HP and GH, both in plasma and in in vitro assay. Similarly, SIRT1 was also reduced in pregnant women who subsequently developed PE (case) compared to women who had healthy pregnancies (control). This reduction may be indicative of possible underlying pathophysiology mechanisms in PE. (AU)

• Processo FAPESP: 08/53593-0 - Fatores etiológicos da prematuridade e conseqüências dos fatores perinatais na saúde da criança: coortes de nascimentos em duas cidades brasileiras
• Beneficiário: Marco Antonio Barbieri
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/06118-0 - Análise da expressão e atividade enzimática de SIRT1 e sua modulação pelo resveratrol em modelo in vitro de Pré-Eclâmpsia
• Beneficiário: Sarah Viana Mattioli
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/20461-8 - Papel do NRF2 e HO-1 em pré-eclâmpsia: estudo de polimorfismos genéticos, fatores circulantes e ensaios in vitro
• Beneficiário: Valeria Cristina Sandrim
• Modalidade de apoio: Auxílio à Pesquisa - Regular