Analysis of SIRT1 Expression in Plasma and in an In Vitro Model of Preeclampsia

Author(s)

Viana-Mattioli, Sarah ^[1] ; Nunes, Priscila ^[2] ; Cavalli, Ricardo ^[3] ; Sandrim, Valeria ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Inst Biosci Botucatu, Dept Pharmacol, BR-18680000 Botucatu, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Med Sch, BR-18680000 Botucatu, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Gynecol & Obstet, BR-14049900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY; v. 2020, APR 28 2020.
• Web of Science Citations: 0

Abstract

Preeclampsia (PE) is a pregnancy-specific disorder that affects 3-8% expecting mothers worldwide being one of the main causes of maternal and fetal morbidity and mortality. The search for altered circulating molecules in PE is an important target to better understand the pathophysiology of this disease. Therefore, we evaluated Sirtuin-1 (SIRT1) concentration in plasma from healthy pregnant (HP) women, gestational hypertensive women (GH), and preeclampsia women (PE) via enzyme-linked immunosorbent assay (ELISA). We also measured intracellular SIRT1 in HUVECs incubated with plasma from PE patients compared to HP and GH via Western Blot Assay. Statistical differences were considered when p<0.05. SIRT1 was downregulated in PE compared to HP and GH, both in plasma and in in vitro assay. Similarly, SIRT1 was also reduced in pregnant women who subsequently developed PE (case) compared to women who had healthy pregnancies (control). This reduction may be indicative of possible underlying pathophysiology mechanisms in PE. (AU)

• FAPESP's process: 08/53593-0 - Etiological factors of preterm birth and consequences of perinatal factors in child health: birth cohorts in two Brazilian cities (BRISA project)
• Grantee: Marco Antonio Barbieri
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/06118-0 - Analysis and the expression of Sirt1 and its modulation for resveratrol in vitro model of Pré-Eclampsia
• Grantee: Sarah Viana Mattioli
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/20461-8 - Role of NRF2 and HO-1 in preeclampsia: polymorphisms, circulating factors and in vitro assays
• Grantee: Valeria Cristina Sandrim
• Support Opportunities: Regular Research Grants