Passion fruit (Passiflora edulis) leaf aqueous extract ameliorates intestinal epithelial barrier dysfunction and reverts inflammatory parameters in Caco-2 cells monolayer

Autor(es)

Lopes do Carmo, Monica Cristina ^[1] ; Martins, Isabela Mateus ^[1] ; Magalhaes, Ana Elisa Ramos ^[1] ; Marostica Junior, Mario Roberto ^[1] ; Macedo, Juliana Alves ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Fac Food Engn, Food & Nutr Dept, Rua Monteiro Lobato 80, Cidade Univ Zeferino Vaz, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 133, JUL 2020.
• Citações Web of Science: 0

Resumo

The aqueous extract of Passiflora edulis leaves is rich in bioactive polyphenolics, with antioxidant and anti-inflammatory properties. In this study, the recovery of barrier dysfunction and the anti-inflammatory effect of P. edulis leaf aqueous extract (PELE) were evaluated using a Caco-2 monolayer model challenged with IL-1 beta and LPS. After inflammatory stimuli, it was observed a 28% reduction in transepithelial electrical resistance (TER) and 78% increase of LY permeability. After 48-h treatment with PELE (10 mg mL(-1)), the monolayer showed 35% increase in TER after inflammatory decreases, and 67% lower LY paracellular permeability, showing a recovery of the monolayer integrity. The treatment also suppressed IL-8 production in 65%. Our results suggest that PELE is a potent source of antioxidants that may promote a protective effect by repairing the intestinal epithelial integrity. (AU)

• Processo FAPESP: 15/50333-1 - Cobra: nova plataforma de descoberta de compostos para identificação rápida e de baixo custo de bioativo com efeitos benéficos originários de plantas brasileiras
• Beneficiário: Glaucia Maria Pastore
• Modalidade de apoio: Auxílio à Pesquisa - Temático