The novel coronavirus SARS-CoV-2: From a zoonotic infection to coronavirus disease 2019

Autor(es)

Bezerra, Rafael dos Santos ^{[1, 2]} ; Valenca, Ian N. ^{[1, 2, 3]} ; Ruy, Patricia de Cassia ^[4] ; Ximenez, Joao P. B. ^[1] ; Silva Junior, Wilson A. ^[5] ; Covas, Dimas T. ^[1] ; Kashima, Simone ^[1] ; Slavov, Svetoslav N. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Lab Mol Biol, Blood Ctr Ribeirao Preto, 2501 Tenente Catao Roxo St, BR-14051060 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Posgrad Program Clin Oncol Stem Cells & Cell Ther, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[3] Silva Junior, Wilson A., Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, Ribeirao Preto, SP, Brazil.Bezerra, Rafael dos Santos, Univ Sao Paulo, Posgrad Program Clin Oncol Stem Cells & Cell Ther, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Ctr Med Genom, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Virology; v. 92, n. 11 JUN 2020.
• Citações Web of Science: 2

Resumo

The novel coronavirus (CoV), severe acute respiratory syndrome (SARS)-CoV-2 is an international public health emergency. Until now, the intermediate host and mechanisms of the interspecies jump of this virus are unknown. Phylogenetic analysis of all available bat CoV complete genomes was performed to analyze the relationships between bat CoV and SARS-CoV-2. To suggest a possible intermediate host, another phylogenetic reconstruction of CoV genomes obtained from animals that were hypothetically commercialized in the Chinese markets was also carried out. Moreover, mutation analysis was executed to suggest genomic regions that may have permitted the adaptation of SARS-CoV-2 to the human host. The phylogenetic analysis demonstrated that SARS-CoV-2 formed a cluster with the bat CoV isolate RaTG13. Possible CoV interspecies jumps among bat isolates were also observed. The phylogenetic tree reconstructed from CoV strains belonging to different animals demonstrated that SARS-CoV-2, bat RaTG13, and pangolin CoV genomes formed a monophyletic cluster, demonstrating that pangolins may be suggested as SARS-CoV-2 intermediate hosts. Three AA substitutions localized in the S1 portion of the S gene were observed, some of which have been correlated to structural modifications of the S protein which may facilitate SARS-CoV-2 tropism to human cells. Our analysis shows the tight relationship between SARS-CoV-2 and bat SARS-like strains. It also hypothesizes that pangolins might have been possible intermediate hosts of the infection. Some of the observed AA substitutions in the S-binding protein may serve as possible adaptation mutations in humans but more studies are needed to elucidate their function. (AU)

• Processo FAPESP: 19/08528-0 - Aplicação de métodos de bioinformática para identificação de infecções virais com impacto em hemoterapia
• Beneficiário: Rafael dos Santos Bezerra
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/15826-5 - Avaliação do impacto de vírus emergentes e re-emergentes em hemoterapia e transplante de células-tronco por meio de técnicas moleculares avançadas
• Beneficiário: Svetoslav Nanev Slavov
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 19/07861-8 - Desenvolvimento de um pipeline de bioinformática para identificação de doenças infecciosas emergentes em pacientes em regime de transfusão crônica
• Beneficiário: Ian Nunes Valença
• Modalidade de apoio: Bolsas no Brasil - Mestrado