Bezerra, Rafael dos Santos
Valenca, Ian N.
[1, 2, 3]
Ruy, Patricia de Cassia
Ximenez, Joao P. B.
Silva Junior, Wilson A.
Covas, Dimas T.
Slavov, Svetoslav N.
Total Authors: 8
 Univ Sao Paulo, Fac Med Ribeirao Preto, Lab Mol Biol, Blood Ctr Ribeirao Preto, 2501 Tenente Catao Roxo St, BR-14051060 Ribeirao Preto, SP - Brazil
 Univ Sao Paulo, Posgrad Program Clin Oncol Stem Cells & Cell Ther, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil
 Silva Junior, Wilson A., Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, Ribeirao Preto, SP, Brazil.Bezerra, Rafael dos Santos, Univ Sao Paulo, Posgrad Program Clin Oncol Stem Cells & Cell Ther, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil
 Univ Sao Paulo, Ctr Med Genom, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil
 Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 5
Journal of Medical Virology;
Web of Science Citations:
The novel coronavirus (CoV), severe acute respiratory syndrome (SARS)-CoV-2 is an international public health emergency. Until now, the intermediate host and mechanisms of the interspecies jump of this virus are unknown. Phylogenetic analysis of all available bat CoV complete genomes was performed to analyze the relationships between bat CoV and SARS-CoV-2. To suggest a possible intermediate host, another phylogenetic reconstruction of CoV genomes obtained from animals that were hypothetically commercialized in the Chinese markets was also carried out. Moreover, mutation analysis was executed to suggest genomic regions that may have permitted the adaptation of SARS-CoV-2 to the human host. The phylogenetic analysis demonstrated that SARS-CoV-2 formed a cluster with the bat CoV isolate RaTG13. Possible CoV interspecies jumps among bat isolates were also observed. The phylogenetic tree reconstructed from CoV strains belonging to different animals demonstrated that SARS-CoV-2, bat RaTG13, and pangolin CoV genomes formed a monophyletic cluster, demonstrating that pangolins may be suggested as SARS-CoV-2 intermediate hosts. Three AA substitutions localized in the S1 portion of the S gene were observed, some of which have been correlated to structural modifications of the S protein which may facilitate SARS-CoV-2 tropism to human cells. Our analysis shows the tight relationship between SARS-CoV-2 and bat SARS-like strains. It also hypothesizes that pangolins might have been possible intermediate hosts of the infection. Some of the observed AA substitutions in the S-binding protein may serve as possible adaptation mutations in humans but more studies are needed to elucidate their function. (AU)