Antibacterial activity of salvia officinalis L. against periodontopathogens: An in vitro study

Autor(es)

Ferreira Mendes, Filipe Santos ^[1] ; Garcia, Leticia Matanovich ^{[1, 2]} ; Moraes, Thais da Silva ^{[1, 2]} ; Casemiro, Luciana Assirati ^{[1, 2]} ; de Alcantara, Clauber Barbosa ^[3] ; Ambrosio, Sergio Ricardo ^[3] ; Sola Veneziani, Rodrigo Cassio ^[3] ; Dantas Miranda, Mayker Lazaro ^[4] ; Gomes Martins, Carlos Henrique ^{[1, 2, 5]}

Afiliação do(s) autor(es)

^[1] Univ Franca, Lab Res Appl Microbiol, Franca, SP - Brazil

^[2] Gomes Martins, Carlos Henrique, Univ Fed Uberlandia, Inst Biomed Sci, Lab Antimicrobial Trials LEA, Av Para,1720,Bloco 2B Sala 221, BR-38405320 Uberlandia, MG, Brazil.Ferreira Mendes, Filipe Santos, Univ Franca, Lab Res Appl Microbiol, Franca, SP - Brazil

^[3] Univ Franca, Nucleus Res Sci & Technol, Franca, SP - Brazil

^[4] Fed Inst Educ Sci & Technol Triangulo Mineiro, Uberlandia, MG - Brazil

^[5] Univ Fed Uberlandia, Inst Biomed Sci, Lab Antimicrobial Trials LEA, Av Para, 1720, Bloco 2B Sala 221, BR-38405320 Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Anaerobe; v. 63, JUN 2020.
• Citações Web of Science: 2

Resumo

Being aware of the remarkable antimicrobial potential of S. officinalis L., we aimed to evaluate the antimicrobial activity of the S. officinalis dichloromethane crude extract (SOD), dichloromethane-soluble fractions (SODH and SODD), SODD subfractions (SODD1 and SODD2), and pure substances (manool, salvigenin, and viridiflorol) against periodontopathogens. This bioassay-guided study comprises five antimicrobial tests-determination of the Minimum Inhibitory Concentration (MIC), determination of the Minimum Bactericidal Concentration (MBC), determination of the antibiofilm activity, construction of the Time-kill curve (determination of Bactericidal Kinetics), and determination of the Fractional Inhibitory Concentration Index-on six clinical bacterial isolates and three standard bacterial strains involved in periodontal disease. SOD has moderate activity against most of the tested bacteria, whereas SODD1, SODH1, SODH3, and manool afford the lowest results. The Porphyromonas gingivalis (ATTC and clinical isolate) biofilm is considerably resistant to all the samples. In association with chlorhexidine gluconate, only SODH1 exerts additive action against P. gingivalis (clinical isolate). Therefore, SODH1 and manool are promising antibacterial agents and may provide therapeutic solutions for periodontal infections. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/12880-2 - Estudo do potencial antibacteriano do extrato bruto e derivados de Salvia officinalis frente a bactérias periodontopatogênicas
• Beneficiário: Filipe Santos Ferreira Mendes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático