Antibacterial activity of salvia officinalis L. against periodontopathogens: An in vitro study

Author(s)

Ferreira Mendes, Filipe Santos ^[1] ; Garcia, Leticia Matanovich ^{[1, 2]} ; Moraes, Thais da Silva ^{[1, 2]} ; Casemiro, Luciana Assirati ^{[1, 2]} ; de Alcantara, Clauber Barbosa ^[3] ; Ambrosio, Sergio Ricardo ^[3] ; Sola Veneziani, Rodrigo Cassio ^[3] ; Dantas Miranda, Mayker Lazaro ^[4] ; Gomes Martins, Carlos Henrique ^{[1, 2, 5]}

Affiliation

^[1] Univ Franca, Lab Res Appl Microbiol, Franca, SP - Brazil

^[2] Gomes Martins, Carlos Henrique, Univ Fed Uberlandia, Inst Biomed Sci, Lab Antimicrobial Trials LEA, Av Para,1720,Bloco 2B Sala 221, BR-38405320 Uberlandia, MG, Brazil.Ferreira Mendes, Filipe Santos, Univ Franca, Lab Res Appl Microbiol, Franca, SP - Brazil

^[3] Univ Franca, Nucleus Res Sci & Technol, Franca, SP - Brazil

^[4] Fed Inst Educ Sci & Technol Triangulo Mineiro, Uberlandia, MG - Brazil

^[5] Univ Fed Uberlandia, Inst Biomed Sci, Lab Antimicrobial Trials LEA, Av Para, 1720, Bloco 2B Sala 221, BR-38405320 Uberlandia, MG - Brazil

• Document type: Journal article
• Source: Anaerobe; v. 63, JUN 2020.
• Web of Science Citations: 2

Abstract

Being aware of the remarkable antimicrobial potential of S. officinalis L., we aimed to evaluate the antimicrobial activity of the S. officinalis dichloromethane crude extract (SOD), dichloromethane-soluble fractions (SODH and SODD), SODD subfractions (SODD1 and SODD2), and pure substances (manool, salvigenin, and viridiflorol) against periodontopathogens. This bioassay-guided study comprises five antimicrobial tests-determination of the Minimum Inhibitory Concentration (MIC), determination of the Minimum Bactericidal Concentration (MBC), determination of the antibiofilm activity, construction of the Time-kill curve (determination of Bactericidal Kinetics), and determination of the Fractional Inhibitory Concentration Index-on six clinical bacterial isolates and three standard bacterial strains involved in periodontal disease. SOD has moderate activity against most of the tested bacteria, whereas SODD1, SODH1, SODH3, and manool afford the lowest results. The Porphyromonas gingivalis (ATTC and clinical isolate) biofilm is considerably resistant to all the samples. In association with chlorhexidine gluconate, only SODH1 exerts additive action against P. gingivalis (clinical isolate). Therefore, SODH1 and manool are promising antibacterial agents and may provide therapeutic solutions for periodontal infections. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 12/12880-2 - Study of the antibacterial potential of the crude extract and derivatives of Salvia officinalis against periodontopathogenic bacteria
• Grantee: Filipe Santos Ferreira Mendes
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants