What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA?

Autor(es)

Colombini-Ishikiriama, Bella Luna ^[1] ; Dionisio, Thiago Jose ^[1] ; Garbieri, Thais Francini ^[1] ; da Silva, Rafaela Alves ^[2] ; Andrade Moreira Machado, Maria Aparecida ^[3] ; Penha de Oliveira, Sandra Helena ^[4] ; Lara, Vanessa Soares ^[2] ; Greene, Andrew Seth ^[5] ; Santos, Carlos Ferreira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Surg Stomatol Pathol & Radiol, Bauru, SP - Brazil

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Bauru, SP - Brazil

^[4] Sao Paulo State Univ, Sch Dent Aracatuba, Dept Basic Sci, Aracatuba, SP - Brazil

^[5] Jackson Lab, 600 Main St, Bar Harbor, ME 04609 - USA

• Tipo de documento: Artigo Científico
• Fonte: BMC IMMUNOLOGY; v. 21, n. 1 JUN 22 2020.
• Citações Web of Science: 0

Resumo

Background Oral fibroblast immunological responses to bacterial stimuli are well known. However, there are few studies about pulp fibroblasts from deciduous teeth (HDPF) responses, which are important for the treatment of pulp infections in children. The aim of this study was to evaluate expression and production of inflammatory cytokines and chemokines by HDPF when challenged with bacterial antigens normally present in advanced caries lesions. Methods Triplicate HDPF from 4 children (n = 4; 2 boys and 2 girls) were cultured by explant technique and challenged or not withEscherichia colilipopolysaccharide/1 mu g/mL(EcLPS)orEnterococcus faecalislipoteichoic acid/1 mu g/mL(EfLTA)for 6 and 24 h. Most of published studies employed immortalized cells, i.e., without checking possible gender and genetic variables. mRNA expression and protein production were evaluated by RT-qPCR and ELISA MILLIPLEX (R), respectively, for Interleukin (IL)-1 alpha, IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, Chemokine C-C motif ligand 2/monocyte chemoattractant protein 1 (CCL2/MCP-1), Chemokine C-C motif ligand 3/macrophage inflammatory protein 1-alpha (CCL3/MIP1-alpha), Chemokine C-C motif ligand 5/ regulated on activation, normal T cell expressed and secreted (CCL5/RANTES), C-X-C motif chemokine 12/ stromal cell-derived factor 1 (CXCL12/SDF-1), Tumor Necrosis Factor-alpha (TNF-alpha), Interferon-gamma (IFN gamma), Vascular Endothelial Growth Factor (VEGF), Colony stimulating factor 1 (CSF-1) and Macrophage colony-stimulating factor (M-CSF). Results EcLPSincreased IL-1 alpha, IL-1 beta, IL-8, CCL2, CCL5, TNF-alpha and CSF-1 mRNA and protein levels whileEfLTAwas only able to positively regulate gene expression and protein production of IL-8. Conclusion The results of the present study confirmed our hypothesis, since pulp fibroblasts from deciduous teeth are capable of increasing gene expression and protein production after being stimulated withEcLPSandEfLTA. (AU)

• Processo FAPESP: 16/11450-5 - Estudo do papel de componentes do sistema renina-angiotensina na imunomodulação de fibroblastos de polpa dental de dentes decíduos.
• Beneficiário: Bella Luna Colombini Ishikiriama
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/03965-2 - Papel do sistema renina-angiotensina em diferentes modelos inflamatórios orais: uma abordagem interdisciplinar experimental e clínica
• Beneficiário: Carlos Ferreira dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Temático