What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA?

Author(s)

Colombini-Ishikiriama, Bella Luna ^[1] ; Dionisio, Thiago Jose ^[1] ; Garbieri, Thais Francini ^[1] ; da Silva, Rafaela Alves ^[2] ; Andrade Moreira Machado, Maria Aparecida ^[3] ; Penha de Oliveira, Sandra Helena ^[4] ; Lara, Vanessa Soares ^[2] ; Greene, Andrew Seth ^[5] ; Santos, Carlos Ferreira ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Surg Stomatol Pathol & Radiol, Bauru, SP - Brazil

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Bauru, SP - Brazil

^[4] Sao Paulo State Univ, Sch Dent Aracatuba, Dept Basic Sci, Aracatuba, SP - Brazil

^[5] Jackson Lab, 600 Main St, Bar Harbor, ME 04609 - USA

• Document type: Journal article
• Source: BMC IMMUNOLOGY; v. 21, n. 1 JUN 22 2020.
• Web of Science Citations: 0

Abstract

Background Oral fibroblast immunological responses to bacterial stimuli are well known. However, there are few studies about pulp fibroblasts from deciduous teeth (HDPF) responses, which are important for the treatment of pulp infections in children. The aim of this study was to evaluate expression and production of inflammatory cytokines and chemokines by HDPF when challenged with bacterial antigens normally present in advanced caries lesions. Methods Triplicate HDPF from 4 children (n = 4; 2 boys and 2 girls) were cultured by explant technique and challenged or not withEscherichia colilipopolysaccharide/1 mu g/mL(EcLPS)orEnterococcus faecalislipoteichoic acid/1 mu g/mL(EfLTA)for 6 and 24 h. Most of published studies employed immortalized cells, i.e., without checking possible gender and genetic variables. mRNA expression and protein production were evaluated by RT-qPCR and ELISA MILLIPLEX (R), respectively, for Interleukin (IL)-1 alpha, IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, Chemokine C-C motif ligand 2/monocyte chemoattractant protein 1 (CCL2/MCP-1), Chemokine C-C motif ligand 3/macrophage inflammatory protein 1-alpha (CCL3/MIP1-alpha), Chemokine C-C motif ligand 5/ regulated on activation, normal T cell expressed and secreted (CCL5/RANTES), C-X-C motif chemokine 12/ stromal cell-derived factor 1 (CXCL12/SDF-1), Tumor Necrosis Factor-alpha (TNF-alpha), Interferon-gamma (IFN gamma), Vascular Endothelial Growth Factor (VEGF), Colony stimulating factor 1 (CSF-1) and Macrophage colony-stimulating factor (M-CSF). Results EcLPSincreased IL-1 alpha, IL-1 beta, IL-8, CCL2, CCL5, TNF-alpha and CSF-1 mRNA and protein levels whileEfLTAwas only able to positively regulate gene expression and protein production of IL-8. Conclusion The results of the present study confirmed our hypothesis, since pulp fibroblasts from deciduous teeth are capable of increasing gene expression and protein production after being stimulated withEcLPSandEfLTA. (AU)

• FAPESP's process: 16/11450-5 - Study of the role of renin-angiotensin system components in the immunomodulation of fibroblasts from deciduous dental pulp teeth.
• Grantee: Bella Luna Colombini Ishikiriama
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach
• Grantee: Carlos Ferreira dos Santos
• Support Opportunities: Research Projects - Thematic Grants