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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Enteric viruses circulating in undiagnosed central nervous system infections at tertiary hospital in Sao Jose do Rio Preto, Sao Paulo, Brazil

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Autor(es):
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da Rocha, Leonardo Cecilio [1] ; Estofolete, Cassia Fernanda [1] ; Goncalves de Aguiar Milhim, Bruno Henrique [1] ; Augusto, Marcos Tayar [1] ; Zini, Nathalia [1] ; Dutra da Silva, Gislaine Celestino [1] ; Ferraz-Junior, Helio Correa [1] ; Sabadoto Brienze, Vania Maria [2] ; Liso, Elisabete [2] ; Cunha, Mariana Sequetin [3] ; Sabino, Ester Cerdeira [4, 5] ; da Costa, Antonio Charlys [4] ; Nogueira, Mauricio Lacerda [1] ; Luchs, Adriana [6] ; Bernardes Terzian, Ana Carolina [1]
Número total de Autores: 15
Afiliação do(s) autor(es):
[1] Sao Jose do Rio Preto Sch Med FAMERP, Av Brigadeiro Faria Lima 5416, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil
[2] Sao Jose do Rio Preto Base Hosp, Sao Paulo - Brazil
[3] Adolfo Lutz Inst, Virol Ctr, Vector Borne Dis Lab, Sao Paulo - Brazil
[4] Univ Sao Paulo, Inst Trop Med, Sao Paulo - Brazil
[5] Univ Sao Paulo, Fac Med, LIM 46, Sao Paulo - Brazil
[6] Adolfo Lutz Inst, Virol Ctr, Enter Dis Lab, Av Dr Arnaldo 355, BR-01246902 Sao Paulo, SP - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: Journal of Medical Virology; JUL 2020.
Citações Web of Science: 0
Resumo

Enterovirus (EV) is commonly associated with central nervous system (CNS) syndromes. Recently, gastroenteric viruses, including rotavirus (RVA), human astrovirus (HAstV), and norovirus (NoV), have also been associated with CNS neurological disorders. The aim of the present study was to investigate the presence of EV, RVA, HAst, and NoV associated to CNS infections with undiagnosed etiology in Northwest region of Sao Paulo State, Brazil, and to conduct the molecular characterization of the positive samples detected. A total of 288 cerebrospinal fluid samples collected from July to December 2017 were tested for EV and NoV by quantitative real-time polymerase chain reaction (RT-qPCR), HAstV by conventional RT-PCR, and RVA by enzyme-linked immunosorbent assay. Positive-EV samples were inoculated in cells lines, amplified by RT-PCR and sequenced. RVA, NoV, and HAstV were not detected. EV infection was detected in 5.5% (16/288), and five samples successful genotyped: echovirus 3 (E3) (1/5), coxsackie virus A6 (CVA6) (1/5), and coxsackie virus B4 (CVB4) (3/5). Meningitis was the main syndrome observed (12/16; 75%). CVA6, CVB4, and E3 were identified associated with aseptic meningitis. Reports of CVA6 associated with aseptic meningitis are rare, E3 had not been previously reported in Brazil, and epidemiological data on CVB4 in the country is virtually unknown. The present investigation illustrates the circulation of diverse EV types in a small regional sample set and in a short period of time, highlighting the importance of an active EV surveillance system in CNS infections. Enhanced understanding of undiagnosed CNS infections will assist in public health and health care planning. (AU)

Processo FAPESP: 17/00021-9 - Metagenômica viral da dengue, Chikungunya e Zika: acompanhar, explicar e prever a transmissão e distribuição espaço-temporal N‚O Brasil
Beneficiário:Antonio Charlys da Costa
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/21719-3 - Estudo epidemiológico da dengue (sorotipos 1 a 4) em coorte prospectiva de São José do Rio Preto, São Paulo, Brasil, durante 2014 a 2018
Beneficiário:Maurício Lacerda Nogueira
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/12295-0 - Diagnóstico de arboviroses brasileiras e emergentes em pacientes e mosquitos em duas regiões distintas do Brasil
Beneficiário:Ana Carolina Bernardes Terzian
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/15021-1 - Estudo clínico-epidemiológico em coorte prospectiva de gestantes infectadas pelo vírus Zika em São José do Rio Preto
Beneficiário:Maurício Lacerda Nogueira
Linha de fomento: Auxílio à Pesquisa - Pesquisa em Políticas Públicas para o SUS