Enteric viruses circulating in undiagnosed central nervous system infections at tertiary hospital in Sao Jose do Rio Preto, Sao Paulo, Brazil

Author(s):Show less -

da Rocha, Leonardo Cecilio ^[1] ; Estofolete, Cassia Fernanda ^[1] ; Goncalves de Aguiar Milhim, Bruno Henrique ^[1] ; Augusto, Marcos Tayar ^[1] ; Zini, Nathalia ^[1] ; Dutra da Silva, Gislaine Celestino ^[1] ; Ferraz-Junior, Helio Correa ^[1] ; Sabadoto Brienze, Vania Maria ^[2] ; Liso, Elisabete ^[2] ; Cunha, Mariana Sequetin ^[3] ; Sabino, Ester Cerdeira ^{[4, 5]} ; da Costa, Antonio Charlys ^[4] ; Nogueira, Mauricio Lacerda ^[1] ; Luchs, Adriana ^[6] ; Bernardes Terzian, Ana Carolina ^[1]

Affiliation

^[1] Sao Jose do Rio Preto Sch Med FAMERP, Av Brigadeiro Faria Lima 5416, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Sao Jose do Rio Preto Base Hosp, Sao Paulo - Brazil

^[3] Adolfo Lutz Inst, Virol Ctr, Vector Borne Dis Lab, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Trop Med, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Med, LIM 46, Sao Paulo - Brazil

^[6] Adolfo Lutz Inst, Virol Ctr, Enter Dis Lab, Av Dr Arnaldo 355, BR-01246902 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Journal of Medical Virology; v. 93, n. 6 JUL 2020.
• Web of Science Citations: 2

Abstract

Enterovirus (EV) is commonly associated with central nervous system (CNS) syndromes. Recently, gastroenteric viruses, including rotavirus (RVA), human astrovirus (HAstV), and norovirus (NoV), have also been associated with CNS neurological disorders. The aim of the present study was to investigate the presence of EV, RVA, HAst, and NoV associated to CNS infections with undiagnosed etiology in Northwest region of Sao Paulo State, Brazil, and to conduct the molecular characterization of the positive samples detected. A total of 288 cerebrospinal fluid samples collected from July to December 2017 were tested for EV and NoV by quantitative real-time polymerase chain reaction (RT-qPCR), HAstV by conventional RT-PCR, and RVA by enzyme-linked immunosorbent assay. Positive-EV samples were inoculated in cells lines, amplified by RT-PCR and sequenced. RVA, NoV, and HAstV were not detected. EV infection was detected in 5.5% (16/288), and five samples successful genotyped: echovirus 3 (E3) (1/5), coxsackie virus A6 (CVA6) (1/5), and coxsackie virus B4 (CVB4) (3/5). Meningitis was the main syndrome observed (12/16; 75%). CVA6, CVB4, and E3 were identified associated with aseptic meningitis. Reports of CVA6 associated with aseptic meningitis are rare, E3 had not been previously reported in Brazil, and epidemiological data on CVB4 in the country is virtually unknown. The present investigation illustrates the circulation of diverse EV types in a small regional sample set and in a short period of time, highlighting the importance of an active EV surveillance system in CNS infections. Enhanced understanding of undiagnosed CNS infections will assist in public health and health care planning. (AU)

• FAPESP's process: 13/21719-3 - Epidemiological study of dengue (serotypes1-4) in a cohort of São José do Rio Preto, São Paulo, Brazil, during 2014-2018
• Grantee: Maurício Lacerda Nogueira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/15021-1 - Clinical-epidemiological study of a cohort of Zika infected pregnant women in São José do Rio Preto, SP
• Grantee: Maurício Lacerda Nogueira
• Support Opportunities: Research Grants - Research in Public Policies for the National Health Care System (PP-SUS)
• FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Antonio Charlys da Costa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/12295-0 - Diagnostic of Brazilian and emergent arboviruses in patients and mosquitoes from two distinct regions in Brazil
• Grantee: Ana Carolina Bernardes Terzian
• Support Opportunities: Scholarships in Brazil - Post-Doctoral