Influence of nano-hydroxyapatite coating implants on gene expression of osteogenic markers andmicro-CTparameters. An in vivo study in diabetic rats

Autor(es)

de Oliveira, Paula Gabriela Faciola Pessoa ^[1] ; de Melo Soares, Mariana Sales ^[1] ; Silveira e Souza, Adriana Maria Mariano ^[1] ; Taba Jr, Mario ; Palioto, Daniela Bazan ^[2] ; Messora, Michel Reis ^[2] ; Ghiraldini, Bruna ^[3] ; Nunes, Felipe Anderson de Sousa ^[2] ; de Souza, Sergio Luis Scombatti ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Oral & Maxillofacial Surg & Periodontol, FORP USP, Ribeirao Preto, SP - Brazil

^[2] Taba Jr, Jr., Mario, Univ Sao Paulo, Dept Oral & Maxillofacial Surg & Periodontol, FORP USP, Ribeirao Preto, SP - Brazil

^[3] Univ Paulista, Sch Dent, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Materials Research Part A; v. 109, n. 5 JUL 2020.
• Citações Web of Science: 2

Resumo

This study evaluated the response of a nano-hydroxyapatite coating implant through gene expression analysis (runt-related transcription factor 2 (Runx2), alkaline phosphatase (Alp), osteopontin (Opn), osteocalcin (Oc), receptor activator of nuclear factor-kappa B (Rank), receptor activator of nuclear factor-kappa B ligand (Rank-L), and osteoprotegerin (Opg)). Three-dimensional evaluation (percent bone volume (BV/TV); percent intersection surface (BIC); bone surface/volume ratio (BS/BV); and total porosity (To.Po)) were also analyzed. Mini implants were surgically placed in tibias of both healthy and diabetic rats. The animals were euthanized at 7 and 30 days. Evaluating all factors the relative expression of Rank showed that NANO surface presented the best results at 7 days (diabetic rats). Furthermore the levels of Runx2, Alp, Oc, and Opn suggest an increase in osteoblasts proliferation, especially in early stages of osseointegration. %BIC in healthy and diabetic (7 days) depicted statistically significant differences for NANO group. BV/TV, BS/BV and To.Po demonstrated higher values for NANO group in all evaluated time point and irrespective of systemic condition, but BS/BV 30 days (healthy rat) and 7 and 30 days (diabetic rat). Microtomographic and gene expression analyses have shown the benefits of nano-hydroxyapatite coated implants in promoting new bone formation in diabetic rats. (AU)

• Processo FAPESP: 16/14584-2 - Teste nanomecânico de uma nova superfície de implante modificada pela adição de nano-hidroxiapatita: estudo in vivo em ratos diabéticos
• Beneficiário: Paula Gabriela Faciola Pessoa de Oliveira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/09879-0 - Avaliação de uma nova superfície de implante com nanotopografia e adição de íons. Estudo in vivo em ratos.
• Beneficiário: Paula Gabriela Faciola Pessoa de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado