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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of nano-hydroxyapatite coating implants on gene expression of osteogenic markers andmicro-CTparameters. An in vivo study in diabetic rats

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Author(s):
de Oliveira, Paula Gabriela Faciola Pessoa [1] ; de Melo Soares, Mariana Sales [1] ; Silveira e Souza, Adriana Maria Mariano [1] ; Taba Jr, Mario ; Palioto, Daniela Bazan [2] ; Messora, Michel Reis [2] ; Ghiraldini, Bruna [3] ; Nunes, Felipe Anderson de Sousa [2] ; de Souza, Sergio Luis Scombatti [2]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Dept Oral & Maxillofacial Surg & Periodontol, FORP USP, Ribeirao Preto, SP - Brazil
[2] Taba Jr, Jr., Mario, Univ Sao Paulo, Dept Oral & Maxillofacial Surg & Periodontol, FORP USP, Ribeirao Preto, SP - Brazil
[3] Univ Paulista, Sch Dent, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Biomedical Materials Research Part A; v. 109, n. 5 JUL 2020.
Web of Science Citations: 2
Abstract

This study evaluated the response of a nano-hydroxyapatite coating implant through gene expression analysis (runt-related transcription factor 2 (Runx2), alkaline phosphatase (Alp), osteopontin (Opn), osteocalcin (Oc), receptor activator of nuclear factor-kappa B (Rank), receptor activator of nuclear factor-kappa B ligand (Rank-L), and osteoprotegerin (Opg)). Three-dimensional evaluation (percent bone volume (BV/TV); percent intersection surface (BIC); bone surface/volume ratio (BS/BV); and total porosity (To.Po)) were also analyzed. Mini implants were surgically placed in tibias of both healthy and diabetic rats. The animals were euthanized at 7 and 30 days. Evaluating all factors the relative expression of Rank showed that NANO surface presented the best results at 7 days (diabetic rats). Furthermore the levels of Runx2, Alp, Oc, and Opn suggest an increase in osteoblasts proliferation, especially in early stages of osseointegration. %BIC in healthy and diabetic (7 days) depicted statistically significant differences for NANO group. BV/TV, BS/BV and To.Po demonstrated higher values for NANO group in all evaluated time point and irrespective of systemic condition, but BS/BV 30 days (healthy rat) and 7 and 30 days (diabetic rat). Microtomographic and gene expression analyses have shown the benefits of nano-hydroxyapatite coated implants in promoting new bone formation in diabetic rats. (AU)

FAPESP's process: 15/09879-0 - Evaluation of a new hydroxiapatite covered nanometric implant surface. An in vitro study in rats
Grantee:Paula Gabriela Faciola Pessoa de Oliveira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/14584-2 - Nanomechanical test of a new 4th generation implant surface, modified by the addition of nano-hydroxyapatite: in vivo study in diabetic rats
Grantee:Paula Gabriela Faciola Pessoa de Oliveira
Support type: Scholarships abroad - Research Internship - Doctorate