Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of nano-hydroxyapatite coating implants on gene expression of osteogenic markers andmicro-CTparameters. An in vivo study in diabetic rats

Full text
Author(s):
de Oliveira, Paula Gabriela Faciola Pessoa [1] ; de Melo Soares, Mariana Sales [1] ; Silveira e Souza, Adriana Maria Mariano [1] ; Taba Jr, Mario ; Palioto, Daniela Bazan [2] ; Messora, Michel Reis [2] ; Ghiraldini, Bruna [3] ; Nunes, Felipe Anderson de Sousa [2] ; de Souza, Sergio Luis Scombatti [2]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Dept Oral & Maxillofacial Surg & Periodontol, FORP USP, Ribeirao Preto, SP - Brazil
[2] Taba Jr, Jr., Mario, Univ Sao Paulo, Dept Oral & Maxillofacial Surg & Periodontol, FORP USP, Ribeirao Preto, SP - Brazil
[3] Univ Paulista, Sch Dent, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Biomedical Materials Research Part A; v. 109, n. 5 JUL 2020.
Web of Science Citations: 2
Abstract

This study evaluated the response of a nano-hydroxyapatite coating implant through gene expression analysis (runt-related transcription factor 2 (Runx2), alkaline phosphatase (Alp), osteopontin (Opn), osteocalcin (Oc), receptor activator of nuclear factor-kappa B (Rank), receptor activator of nuclear factor-kappa B ligand (Rank-L), and osteoprotegerin (Opg)). Three-dimensional evaluation (percent bone volume (BV/TV); percent intersection surface (BIC); bone surface/volume ratio (BS/BV); and total porosity (To.Po)) were also analyzed. Mini implants were surgically placed in tibias of both healthy and diabetic rats. The animals were euthanized at 7 and 30 days. Evaluating all factors the relative expression of Rank showed that NANO surface presented the best results at 7 days (diabetic rats). Furthermore the levels of Runx2, Alp, Oc, and Opn suggest an increase in osteoblasts proliferation, especially in early stages of osseointegration. %BIC in healthy and diabetic (7 days) depicted statistically significant differences for NANO group. BV/TV, BS/BV and To.Po demonstrated higher values for NANO group in all evaluated time point and irrespective of systemic condition, but BS/BV 30 days (healthy rat) and 7 and 30 days (diabetic rat). Microtomographic and gene expression analyses have shown the benefits of nano-hydroxyapatite coated implants in promoting new bone formation in diabetic rats. (AU)

FAPESP's process: 15/09879-0 - Evaluation of a new hydroxiapatite covered nanometric implant surface. An in vitro study in rats
Grantee:Paula Gabriela Faciola Pessoa de Oliveira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/14584-2 - Nanomechanical test of a new 4th generation implant surface, modified by the addition of nano-hydroxyapatite: in vivo study in diabetic rats
Grantee:Paula Gabriela Faciola Pessoa de Oliveira
Support type: Scholarships abroad - Research Internship - Doctorate