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Evaluation of osteogenic differentiation of mesenchymal progenitor cells on the MTA

Grant number: 14/13750-0
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): January 15, 2015
Effective date (End): January 14, 2016
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal researcher:João Eduardo Gomes Filho
Grantee:Índia Olinta de Azevedo Queiroz
Supervisor abroad: Ivo Kalajzic
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Research place: University of Connecticut (UCONN), United States  
Associated to the scholarship:13/06641-8 - Comparative assessment of tissue response in diabetic rats to MTA grey and white MTA, BP.DR


The aim of the study will be to evaluate in vitro and in vivo the influence of MTA on osteogenic differentiation of mesenchymal progenitor cells within bone marrow stromal cells (BMSCs) and periodontal progenitor cells (PDSCs). In addition to standard evaluation for osteogenic differentiation we will utilize osteoprogenitor lineage stage-specific transgenic models. Smooth muscle ±-smooth muscle actin-RFP (±-SMARFP) will be used as marker of progenitor cells while collagen type I (Col2.3GFP) will identify mature osteoblasts/osteocytes. This study will be performed in 2 phases: in vivo and in vitro. For the in vitro assay, BMSC and PDSC derived from ±-SMARFP/Col2.3GFP mice will be isolated, cultured and effects of MTA (Mineral trioxide aggregate) on their osteogenic differentiation investigated for 2 weeks. Expression of ±-SMARFP (progenitor marker) and Col2.3GFP (mature osteoblast marker) will be used to evaluate the osteogenic differentiation. In addition, von Kossa silver nitrate staining, alkaline phosphatase (ALP) activity, alizarin red staining, gene expression for osteocalcin, bone sialoprotein, osterix, runx-2, type I collagen will be evaluated. In vivo assay will be performed by intraosseous implants of MTA and Zinc Oxide and Eugenol (used as control of material) in tibia of eighteen ±-SMARFP/Col2.3GFP mice. Following 21 days, the mice will be sacrificed and the analysis for GFP expression by epifluorescence, (±-SMA; Col2.3GFP) and RT-PCR for osteocalcin, bone sialoprotein, osterix, runx-2, type I collagen will be completed. Extent of new vascularization will be evaluated by immunohistochemistry for CD31. The results will provide critical information on the effects of the MTA on the osteogenic differentiation of mesenchymal progenitor cells. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VIDOVIC ZDRILIC, I.; DE AZEVEDO QUEIROZ, I. O.; MATTHEWS, B. G.; GOMES-FILHO, J. E.; MINA, M.; KALAJZIC, I. Mineral trioxide aggregate improves healing response of periodontal tissue to injury in mice. JOURNAL OF PERIODONTAL RESEARCH, v. 52, n. 6, p. 1058-1067, DEC 2017. Web of Science Citations: 4.

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