Effect of a conditioned medium by bioactive glasses incorporated with protein nanotubes in osteoblast adhesion, proliferation and differentiation

• Grant number: 21/14827-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): March 01, 2022
• Effective date (End): December 31, 2022
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Emanuela Prado Ferraz
• Grantee: Julia de Santana Rodrigues Velho
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Bone defect treatments remain a challenge faced by professionals from different areas. Among the therapeutic strategies, the use of biomaterials associated or not with cells and/or growth factors Isa promising therapy. Up to now, no one material is suitable for use as a bone substitute, and the association of biomaterials has been investigated. In this context, our research group has been studying the effect of a bioglass (BG) incorporated with protein nanotubes (NT) in bone repair. The known bioactivity of BV is related to ionic exchanges with the surrounding environment, resulting in an initial change in ph, which can cause cell damage. To alleviate it, the literature suggests that these materials must undergo a pre-incubation process before ger in contact with cells/tissues. Thus, it is crucial to investigate the cell's contact with the conditioned media under different conditions. This study aims to evaluate the effect of BG-NT conditioned medium with or without pre-incubation on bone marrow-derived mesenchymal stem cell viability and differentiation. For this, BG and BG-NT discs will be pre-incubated in a culture medium for 24 and 48 hours. The ion concentration will be evaluated by plasma by inductive coupling in ppm. Next, cells will be cultivated in an osteogenic medium conditioned by BG or BG-NT with or without pre-incubation. Cell morphology and adhesion will be evaluated by fluorescence), cell proliferation by MTT, and cell differentiation by phosphatase activity alkaline and production of mineralized extracellular matrix. Data will be evaluated using appropriate statistical tests, and the significance will be set as 5%.(AU)