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Comparative assessment of tissue response in diabetic rats to MTA grey and white MTA

Grant number: 13/06641-8
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2013
Effective date (End): February 28, 2017
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:João Eduardo Gomes Filho
Grantee:Índia Olinta de Azevedo Queiroz
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Associated scholarship(s):14/13750-0 - Evaluation of osteogenic differentiation of mesenchymal progenitor cells on the MTA, BE.EP.DR


The aim of this study is to evaluate the relation between diabetes mellitus, tissue response and the mineralization ability of sealers Gray MTA Angelus®, White MTA Angelus®. Zinc Oxide and Eugenol® will be used as a reference of less biocompatible cement. Twenty-four rats will be divided into two groups of twelve animals each: group 1: normal rats, group 2: diabetics rats induced by Alloxan. All animals receive four implants of polyethylene tubes, three containing sealers to be tested and an empty tube for control. At 7, 14 and 21 days will be carried out injections of fluorochromes (Calcein, Alizarin and Oxytretacycline) .After 7 and 30 days, six animals of each group will be sacrificed and the tubes with surrounding tissues removed and fixed. The tubes will be cut in half and a portion processed and included in glycol methacrylate, which are observed in HE staining, Von Kossa, without staining for analysis with polarized light and fluorescence. Tissue reactions on the opening of the tube in contact with the material will receive scores as 0, none or few inflammatory cells and no reaction; 1, less than 25 cells and mild reaction; 2, between 25 and 125 cells and moderate reaction; and 3, 125 and more cells and severe reaction. Fibrous capsule will be considered thin when the thickness be <150 µm and thick at >150 µm. Calcification will be recorded as present or absent. The other half of the tubes will be placed in paraffin for immunohistochemical analysis for the presence of Osteocalcin, Osteopontin and Runx-2. The evaluation criteria will immunostaining: high (near by 3/4 of the cells immunoreactive (IR) per microscopic field), moderate (near by 1/2 IR cells), low (near by 1/4 cell IR) and null (absence IR cells). The results will be analyzed by ANOVA and Kruskal Wallis tests (± = 0.05). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE AZEVEDO QUEIROZ, I. O.; MELLO, W. G.; MARTINS, C. M.; DAL FABBRO, R.; NARCISO, L. G.; MASSUNARI, L.; CINTRA, L. T. A.; ERVOLINO, E.; GOMES-FILHO, J. E.. Systemic bone marker expression induced by grey and white mineral trioxide aggregate in normal and diabetic conditions. International Endodontic Journal, v. 51, n. 8, p. 889-900, . (13/06641-8)
DE AZEVEDO QUEIROZ, INDIA O.; GONCALVES, LUANNA O.; TAKAMIYA, ALINE S.; REZENDE, GABRIELY C.; MACHADO, THIAGO; SIVEIRI-ARAUJO, GUSTAVO; ERVOLINO, EDILSON; OLIVEIRA, SANDRA H. P.; GOMES-FILHO, JOAO E.. Hyperglycemic condition influence on mineral trioxide aggregate biocompatibility and biomineralization. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 108, n. 3, p. 663-673, . (14/25475-4, 13/06641-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
QUEIROZ, Índia Olinta de Azevedo. Analysis of biological properties of MTA in normal and hyperglycemic conditions. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.

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