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Influence of hypertension in tissue response and mineralization capacity of cement repairers MTA Angelus® white and grey and IRM®. Histology and immunohistochemistry analysis in rats.

Grant number: 13/09446-1
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2013
Effective date (End): July 31, 2015
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:João Eduardo Gomes Filho
Grantee:Christine Men Martins
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Associated scholarship(s):14/10541-1 - Osteoclast differentiation, nodule formation, general bone and periapical lesion phenotype in BPH/2 strain mice, BE.EP.DR


A hypertensive state may cause a greater mobilization of calcium from bone and its increased excretion by the kidneys. Angelus MTA ® is a repairman sealer that induces mineralization in tissues where it is applied. Therefore, it can be inferred that the tissues healing after endodontic treatment in hypertensive people with this cement may be impaired. Thus, the objective of this study is to evaluate the relationship between hypertension, tissue response and the ability of mineralization of sealers repairers Angelus MTA ® White and Grey and IRM ®, through an experimental rat model. Will be used 16 hypertensive rats and 16 normotensive rats. All animals receive four polyethylene tubes implants, three containing the cements to be tested and one empty tube as a control. Within each group, there will be a subdivision, in which 8 animals in each group will be sacrificed on the seventh day and in the remaining animals, will be applied injections of fluorochromes (calcein, alizarin and oxytetracycline), at 7, 14 and 21 days, these being sacrificed to thirtieth day. After sacrificing the animals, polyethylene tubes together with the surrounding tissue will be removed and half the parts will be fixed in glycol methacrylate and processed for histological analysis by hematoxylin and eosin (HE), Von Kossa and without staining for light polarization and fluorescence examination. The evaluation criteria will be 0: no or few cells and no reaction, 1: less than 25 cells and mild reaction; 2: between 25 and 125 cells and moderate reaction, 3: 125 or more cells and severe reaction. Fibrous capsule will be considered thin when <150¼m and thick when> 150¼m. Presence of mineralization will be recorded as present or absent. The other half of the pieces will be analyzed by immunohistochemistry using the specific markers Runx-2, osteocalcin and osteopontin. The results will be tabulated and statistically analyzed by standardizing the level of significance of 5%.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARTINS, C. M.; DE AZEVEDO QUEIROZ, I. O.; ERVOLINO, E.; CINTRA, L. T. A.; GOMES-FILHO, J. E.. RUNX-2, OPN and OCN expression induced by grey and white mineral trioxide aggregate in normal and hypertensive rats. International Endodontic Journal, v. 51, n. 6, p. 641-648, . (13/09446-1)
MARTINS, CHRISTINE MEN; SASAKI, HAJIME; HIRAI, KIMITO; ANDRADA, ANA CRISTINA; GOMES-FILHO, JOAO EDUARDO. Relationship between hypertension and periapical lesion: an in vitro and in vivo study. Brazilian Oral Research, v. 30, . (13/09446-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MARTINS, Christine Men. Influence of hypertension in periapical lesion phenotype pattern, in osteoclastic differentiation, in tissue response and mineralization capacity of MTA repairing cement. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.

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