A hypertensive state may cause a greater mobilization of calcium from bone and its increased excretion by the kidneys. Angelus MTA ® is a repairman sealer that induces mineralization in tissues where it is applied. Therefore, it can be inferred that the tissues healing after endodontic treatment in hypertensive people with this cement may be impaired. Thus, the objective of this study is to evaluate the relationship between hypertension, tissue response and the ability of mineralization of sealers repairers Angelus MTA ® White and Grey and IRM ®, through an experimental rat model. Will be used 16 hypertensive rats and 16 normotensive rats. All animals receive four polyethylene tubes implants, three containing the cements to be tested and one empty tube as a control. Within each group, there will be a subdivision, in which 8 animals in each group will be sacrificed on the seventh day and in the remaining animals, will be applied injections of fluorochromes (calcein, alizarin and oxytetracycline), at 7, 14 and 21 days, these being sacrificed to thirtieth day. After sacrificing the animals, polyethylene tubes together with the surrounding tissue will be removed and half the parts will be fixed in glycol methacrylate and processed for histological analysis by hematoxylin and eosin (HE), Von Kossa and without staining for light polarization and fluorescence examination. The evaluation criteria will be 0: no or few cells and no reaction, 1: less than 25 cells and mild reaction; 2: between 25 and 125 cells and moderate reaction, 3: 125 or more cells and severe reaction. Fibrous capsule will be considered thin when <150¼m and thick when> 150¼m. Presence of mineralization will be recorded as present or absent. The other half of the pieces will be analyzed by immunohistochemistry using the specific markers Runx-2, osteocalcin and osteopontin. The results will be tabulated and statistically analyzed by standardizing the level of significance of 5%.
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