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Biocompatibility and mineralization capacity of tricalcium silicate-based cements: in vitro and in vivo study

Grant number: 14/13353-1
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2014
Effective date (End): March 31, 2016
Field of knowledge:Health Sciences - Dentistry - Endodontics
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Mario Tanomaru Filho
Grantee:Aline Silva Andrade
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


The silicate-based cements have earned highlight. For this purpose, human osteoblast-like cells SAOS-2 (ATCC HTB-85) are used for analysis of cell cytotoxicity by MTT assay and bioactivity by the enzyme alkaline phosphatase activity and the presence of calcium deposits by alizarin red stain. Moreover, the tissue reaction caused by different materials in subcutaneous of sixty rat will be evaluated. Polyethylene tubes filled with materials (tricalcium silicate + 20% zirconium oxide, MTA Plus) will be implanted and empty polyethylene tubes are used as control. After periods of 7, 15, 30 and 60 days, the animals will be euthanized with anesthetic overdose and the connective tissue adjacent to implant will be removed, fixed and embedded in paraffin. Some sections of the subcutaneous tissue will be adhered to glass slides and stained with Carazzi´s hematoxylin and eosin (H & E) to perform the morphological and morphometric analysis of the number of inflammatory cells/mm2. Other sections adhered to silanized slides will be used to perform the immunohistochemical reactions for the detection of interleukin-6 and osteocalcin. The von Kossa method is performed to identify possible calcified structures in the capsule adjacent to implants. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TANOMARU-FILHO, M.; ANDRADE, A. S.; RODRIGUES, E. M.; VIOLA, K. S.; FARIA, G.; CAMILLERI, J.; GUERREIRO-TANOMARU, J. M.. Biocompatibility and mineralized nodule formation of Neo MTA Plus and an experimental tricalcium silicate cement containing tantalum oxide. International Endodontic Journal, v. 50, n. 2, SI, p. E31-E39, . (14/13353-1, 15/03437-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ANDRADE, Aline Silva. Biocompatibility and bioactivity of tricalcium silicate- based cements: in vitro and in vivo studies. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.

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