Evaluation of new substances for root canal irrigation and calcium silicate-based endodontic sealers

• Grant number: 17/14305-9
• Support Opportunities: Regular Research Grants
• Duration: February 01, 2018 - April 30, 2020
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Juliane Maria Guerreiro Tanomaru
• Grantee: Juliane Maria Guerreiro Tanomaru
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Mario Tanomaru Filho

Abstract

Chemical substances are studied as an alternative or in association with the use of sodium hypochlorite (NaOCl), with the aim of promoting cleaning and disinfection of the root canal system (SCR). Calcium Silicate (CS) is the current perspective for the development of new endodontic sealers. This project aims to study new irrigation solutions and to develop new proposals for endodontic calcium silicate (CS) sealers.Subproject 1- aims to evaluate properties of solutions based on calcium hypochlorite (Ca (OCl) 2) and octenidine hydrochloride (OCT): organic tissue dissolution ability, cytotoxicity, antimicrobial activity and against endotoxins (LPS-lipopolysaccharides) with LPS antigenic potential by using the analysis of inflammatory cytokines (IL-1, IL-6 and TNF-±) from mice.Sub-project 2- aims the development of experimental sealer (ES) based on tricalcium silicate, dicalcium silicate and calcium phosphate, handled with different vehicles. These materials will be subjected to the tests of setting time, pH, radiopacity, solubility and flow, and the sealers that present the best results will be compared to TotalFill BC Sealer (TFBC) and AH Plus (AHP) by using conventional tests and volumetric changes in Micro CT, and the antimicrobial activity. The best experimental sealer (ES) and TFBC will be evaluated with or without association with the substance selected in subproject 1 by using physicochemical, antimicrobial, cytotoxicity and cell bioactivity tests. Biocompatibility and bioactivity in vivo will be assessed by subcutaneous analysis in rats. The CE that shows the best results will be selected.Sub-Project 3- aims to evaluate the flow, filling and penetrability of ES (selected in sub-project 2), TFBC, Neo MTA Plus (NMTAP) compared to AHP cement. The flow will be evaluated according to ISO 6876, and additional evaluation in computerized microtomography (Micro-CT). The filling capacity will be evaluated through Micro-CT analysis. In order to evaluate the penetrability of the cements inside the dentinal tubules, bovine teeth prepared and filled with the cements manipulated with B-rhodium for confocal laser scanning microscope (CLSM) evaluation will be used. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMPI, LIVIA BUENO; ESTEVES TORRES, FERNANDA FERRARI; RODRIGUES, ELISANDRA MARCIA; GUERREIRO-TANOMARU, JULIANE MARIA; TANOMARU-FILHO, MARIO. hysicochemical and biological properties of new tricalcium silicate-based repair material doped with fluoride ions and zirconium oxide as radiopacifie. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 110, n. 4, NOV 2021. (18/25364-9, 17/14305-9)

SILVA, E. C. A.; TANOMARU-FILHO, M.; SILVA, G. F.; LOPES, C. S.; CERRI, P. S.; GUERREIRO TANOMARU, J. M.. Evaluation of the biological properties of two experimental calcium silicate sealers: anin vivostudy in rats. International Endodontic Journal, v. 54, n. 1, p. 100-111, JAN 2021. (17/14305-9, 17/19049-0)

CHAVEZ-ANDRADE, GISSELLE MORAIMA; TANOMARU-FILHO, MARIO; BASSO BERNARDI, MARIA INES; LEONARDO, RENATO DE TOLEDO; FARIA, GISELE; GUERREIRO-TANOMARU, JULIANE MARIA. Antimicrobial and biofilm anti-adhesion activities of silver nanoparticles and farnesol against endodontic microorganisms for possible application in root canal treatment. ARCHIVES OF ORAL BIOLOGY, v. 107, NOV 2019. (12/11362-8, 17/14305-9)

CRISTIANE LOPES ZORDAN-BRONZEL; MARIO TANOMARU-FILHO; GISSELLE MORAIMA CHÁVEZ-ANDRADE; FERNANDA FERRARI ESTEVES TORRES; GISELLE PRISCILLA CRUZ ABI-RACHED; JULIANE MARIA GUERREIRO-TANOMARU. Calcium Silicate-Based Experimental Sealers: Physicochemical Properties Evaluation. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 24, n. 1, 2021-01-29. (17/14305-9, 17/19049-0)

ZORDAN-BRONZEL, CRISTIANE LOPES; ESTEVES TORRES, FERNANDA FERRARI; TANOMARU-FILHO, MARIO; CHAVEZ-ANDRADE, GISSELLE MORAIMA; BOSSO-MARTELO, ROBERTA; GUERREIRO-TANOMARU, JULIANE MARIA. Evaluation of Physicochemical Properties of a New Calcium Silicate-based Sealer, Bio-C Sealer. JOURNAL OF ENDODONTICS, v. 45, n. 10, p. 1248-1252, OCT 2019. (17/14305-9, 16/00321-0, 17/19049-0)

QUEIROZ, MARCELA BORSATTO; ESTEVES TORRES, FERNANDA FERRARI; RODRIGUES, ELISANDRA MARCIA; VIOLA, KENNIA SCAPIN; BOSSO-MARTELO, ROBERTA; CHAVEZ-ANDRADE, GISSELLE MORAIMA; GUERREIRO-TANOMARU, JULIANE MARIA; TANOMARU-FILHO, MARIO. Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers. Dental Materials, v. 37, n. 2, p. 311-320, FEB 2021. (16/00321-0, 17/19049-0, 16/11036-4, 17/14305-9)

AIRTON OLIVEIRA SANTOS-JUNIOR; MÁRIO TANOMARU-FILHO; JÁDER CAMILO PINTO; KARINA INES MEDINA CARITA TAVARES; FERNANDA FERRARI ESTEVES TORRES; JULIANE MARIA GUERREIRO-TANOMARU. Effect of obturation technique using a new bioceramic sealer on the presence of voids in flattened root canals. Brazilian Oral Research, v. 35, 2021-02-12. (17/14305-9, 19/22885-0, 16/00321-0, 17/19049-0)

TORRES, F. F. E.; ZORDAN-BRONZEL, C. L.; GUERREIRO-TANOMARU, J. M.; CHAVEZ-ANDRADE, G. M.; PINTO, J. C.; TANOMARU-FILHO, M.. Effect of immersion in distilled water or phosphate-buffered saline on the solubility, volumetric change and presence of voids within new calcium silicate-based root canal sealers. International Endodontic Journal, OCT 2019. (17/14305-9, 16/00321-0, 17/19049-0)

ZORDAN-BRONZEL, C. L.; TANOMARU-FILHO, M.; RODRIGUES, E. M.; CHAVEZ-ANDRADE, G. M.; FARIA, G.; GUERREIRO-TANOMARU, J. M.. Cytocompatibility, bioactive potential and antimicrobial activity of an experimental calcium silicate-based endodontic sealer. International Endodontic Journal, v. 52, n. 7, p. 979-986, JUL 2019. (17/14305-9)