Among the factors that interfere in the process of periapical tissue repair after endodontic treatment, the obturator material, which is in close contact with the periapical tissues, plays a key role in the repair process of this region. An essential property is the biocompatibility that, once not respected, could trigger the occurrence of unfavorable histopathological events. In addition to biocompatible, some materials have the ability to participate in the repair process inducing biomineralization. Considering the development of a bioceramic experimental cement containing calcium hydroxide in its formulation, we propose to quantitatively and qualitatively evaluate its tissue response, comparing it to the Fillapex MTA and the AH Plus. The tissue response will be evaluated according to the norms for tissue biocompatibility tests (ISO 10993-6: 2007). It will be used 160 polyethylene tubes and 40 rats (4 tubes per mouse) divided into 4 groups: G1 - control (empty tubes); G2 - tubes containing the experimental bioceramic cement; G3 - tubes containing the MTA Fillapex cement, G4 - tubes containing the AH Plus. The evaluation periods will be 7, 15, 30, 60 and 90 days. After each postoperative period, eight animals will be sacrificed and the polyethylene tubes with the surrounding tissue will be removed, fixed in 10% formalin solution. The specimens will be processed for light microscopy analysis, including methacrylate glycol, serial cuts of 3¼m and staining by Hematoxylin, serial cuts of 10¼m and staining by Von Kossa and serial cuts of 10 ¼m without staining for analysis under polarized light. Tissue sections will be evaluated by pre-established criteria and scores will be assigned to observed microscopic events. The data obtained will be statistically analyzed by the Kruskall Wallis and Dunn tests with a significance level of 5% (p <0.05).
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