| Texto completo | |
| Autor(es): |
dos Santos Gomes, A. C.
[1]
;
Casciatori, F. P.
[2]
;
Gomes, E.
[1]
;
da Costa Carreira Nunes, C.
[1]
;
Moretti, M. M. S.
[1]
;
Thomeo, J. C.
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Paulista, UNESP, Inst Biociencias Letras & Ciencias Exatas, Rua Cristovao Colombo 2265, Sao Jose Do Rio Preto, SP - Brazil
[2] Fed Univ Sao Carlos UFSCar, Grad Program Chem Engn, Chem Engn Dept, Rodovia Washington Luiz Km 235, BR-13565905 Sao Carlos, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Applied Microbiology; v. 130, n. 1 JUL 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Aims This work aimed to estimate the growth ofMyceliophthora thermophilaM.7 center dot 7 in solid-state cultivation (SSC) through quantification ofN-acetyl-d-glucosamine (NAG) and enzyme activity. Methods and Results The fungus was cultivated in sugarcane bagasse and wheat bran. A consistent statistical analysis was done to assess the reliability of experimental data. Logistic model equation was fitted to experimental data and growth parameters were estimated. The results showed strong influence of the sample size on NAG and a minimum recommended sample size was identified. Scanning electron microscopy (SEM) was used to identify the strategy of substrate colonization. Wheat bran was attacked firstly, while sugarcane bagasse was consumed after wheat bran depletion. The biomass growth was poorly estimated by secretion kinetics of alpha-amylase, endoglucanase, protease and xylanase, but enzyme kinetics were important for understanding substrate colonization. Conclusions In conclusion, the NAG concentration was strongly affected by the sample size and sampling procedure. The strategy of fungal colonization on the substrates was well characterized through SEM analysis. The colonization strategy has direct influence on the kinetic parameters of the logistic model.Myceliophthora thermophilahas a well-defined dynamic of enzyme secretion to degrade the substrate, although the kinetics of enzyme secretion has shown not adequate to characterize the kinetics of fungal growth. Significance and Impact of the Study The paper provides reliable growth kinetic parameters in the SSC of the cellulase producer fungusM. thermophilaM.7 center dot 7, as well as a robust analysis on three indirect methods (NAG, enzymes and SEM) for estimation of fungal development. (AU) | |
| Processo FAPESP: | 14/23453-3 - Ampliação de escala de biorreatores de leito empacotado para a produção de celulases por fermentação em estado sólido |
| Beneficiário: | João Cláudio Thoméo |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Regular |
| Processo FAPESP: | 18/00996-2 - Desenvolvimento de um biorreator de leito empacotado pseudo-contínuo para fermentação em estado sólido |
| Beneficiário: | Fernanda Perpétua Casciatori |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Regular |
| Processo FAPESP: | 10/12624-0 - Aplicação de métodos físico-químicos e enzimáticos na sacarificação do bagaço de cana: estudos de microrganismos, processos fermentativos relacionados e métodos de hidrólise |
| Beneficiário: | Eleni Gomes |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |