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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Growth kinetics ofMyceliophthora thermophilaM.7 center dot 7 in solid-state cultivation

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Author(s):
dos Santos Gomes, A. C. [1] ; Casciatori, F. P. [2] ; Gomes, E. [1] ; da Costa Carreira Nunes, C. [1] ; Moretti, M. M. S. [1] ; Thomeo, J. C. [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, UNESP, Inst Biociencias Letras & Ciencias Exatas, Rua Cristovao Colombo 2265, Sao Jose Do Rio Preto, SP - Brazil
[2] Fed Univ Sao Carlos UFSCar, Grad Program Chem Engn, Chem Engn Dept, Rodovia Washington Luiz Km 235, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Applied Microbiology; v. 130, n. 1 JUL 2020.
Web of Science Citations: 0
Abstract

Aims This work aimed to estimate the growth ofMyceliophthora thermophilaM.7 center dot 7 in solid-state cultivation (SSC) through quantification ofN-acetyl-d-glucosamine (NAG) and enzyme activity. Methods and Results The fungus was cultivated in sugarcane bagasse and wheat bran. A consistent statistical analysis was done to assess the reliability of experimental data. Logistic model equation was fitted to experimental data and growth parameters were estimated. The results showed strong influence of the sample size on NAG and a minimum recommended sample size was identified. Scanning electron microscopy (SEM) was used to identify the strategy of substrate colonization. Wheat bran was attacked firstly, while sugarcane bagasse was consumed after wheat bran depletion. The biomass growth was poorly estimated by secretion kinetics of alpha-amylase, endoglucanase, protease and xylanase, but enzyme kinetics were important for understanding substrate colonization. Conclusions In conclusion, the NAG concentration was strongly affected by the sample size and sampling procedure. The strategy of fungal colonization on the substrates was well characterized through SEM analysis. The colonization strategy has direct influence on the kinetic parameters of the logistic model.Myceliophthora thermophilahas a well-defined dynamic of enzyme secretion to degrade the substrate, although the kinetics of enzyme secretion has shown not adequate to characterize the kinetics of fungal growth. Significance and Impact of the Study The paper provides reliable growth kinetic parameters in the SSC of the cellulase producer fungusM. thermophilaM.7 center dot 7, as well as a robust analysis on three indirect methods (NAG, enzymes and SEM) for estimation of fungal development. (AU)

FAPESP's process: 14/23453-3 - Packed-bed solid-state fermentation bioreactor scale-up for cellulase production
Grantee:João Cláudio Thoméo
Support type: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 18/00996-2 - Development of a pseudo-continuous packed-bed bioreactor for solid-state fermentation
Grantee:Fernanda Perpétua Casciatori
Support type: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 10/12624-0 - Application of physical-chemical and enzymatic methods in the saccharification of sugar cane bagasse: studies on microorganisms, fermentative processes and hydrolyses methods
Grantee:Eleni Gomes
Support type: Research Projects - Thematic Grants