Salivary Microbiological and Gingival Health Status Evaluation of Adolescents With Overweight and Obesity: A Cluster Analysis

Autor(es)

Araujo, Darlle Santos ^[1] ; Klein, Marlise Inez ^[2] ; Scudine, Kelly Guedes de Oliveira ^[1] ; de Sales Leite, Luana ^[2] ; Parisotto, Thais M. ^[3] ; Ferreira, Caroline Marcantonio ^[4] ; Fonseca, Fernando Luiz Affonso ^[4] ; Perez, Matheus Moreira ^[5] ; Castelo, Paula Midori ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas UNICAMP, Dept Pediat Dent, Piracicaba - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

^[3] Sao Francisco Univ, Lab Mol Biol Microorganisms, Braganca Paulista, SP - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Dept Pharmaceut Sci, Diadema - Brazil

^[5] ABC Med Sch, Dept Pathol, Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PEDIATRICS; v. 8, JUL 31 2020.
• Citações Web of Science: 0

Resumo

Given the high prevalence of obesity in children and adolescents, the investigation of early markers is of clinical importance to better manage this condition. Thus, the aim was to evaluate the cross-sectional relationship between salivary microbiota, gingival health status, and excess weight in adolescents. A total of 248 students (14-17 y; 119 girls) were included, free of caries lesions and periodontal pockets. Physical examination included measures of height, weight, and body fat percentage (%BF). Oral examination was performed to gather information on dental (DMFT index) and gingival health status. Unstimulated saliva was submitted to qPCR reactions to quantifyStreptococcus mutans, Porphyromonas gingivalis, Bifidobacteria, andStreptococcus pneumoniaepercentages and the NFKappaB expression. Two-way ANOVA was applied consideringgroup(normal-weight/overweight/obesity) andsexfactors, in addition to cluster analysis.Groupeffect was significant for %S. mutans(partial eta(2)= 0.20;p< 0.001) and %Bifidobacteria (partial eta(2)= 0.19;p< 0.001), with overweight and obesity groups showing the highest levels compared to normal-weight ones, with no significantsexeffect. There was no difference in the frequency of gingivitis,P. gingivalis, andS. pneumoniaepercentages or NFKappaB expression between groups. Cluster analysis generated three clusters according to body fat accumulation: ``Higher %BF,{''} ``Moderate %BF,{''} and ``Lower %BF.{''} ``Higher %BF{''} cluster was characterized by higher body fat percentage and higher salivary %Bifidobacteria, while cluster ``Lower %BF{''} was characterized by lower body fat percentage and lower frequency of gingivitis ({''}Moderate %BF{''} cluster was the contrast). According to nutritional status, a difference in salivaryS. mutansand Bifidobacteria percentages was found, with overweight or obesity adolescents showing the highest percentages than normal-weight ones. Besides, a positive relationship between body fat accumulation and Bifidobacteria count was observed, indicating a possible interaction between oral bacteria communities and weight gain. (AU)

• Processo FAPESP: 14/24804-4 - Avaliação da composição salivar e da qualidade da função mastigatória de adolescentes com sobrepeso e obesidade
• Beneficiário: Paula Midori Castelo Ferrua
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/26400-6 - Impacto da gastroplastia na composição salivar e na citomorfologia da mucosa oral: acompanhamento de três e seis meses
• Beneficiário: Paula Midori Castelo Ferrua
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/08021-5 - Análise da expressão de genes de Streptococcus mutans em biofilme misto
• Beneficiário: Luana de Sales Leite
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico