GSVAscore reveals molecular signatures from transcriptomes for biomaterials comparison

Autor(es)

Ferreira, Marcel R. ^[1] ; Santos, Gerson A. ^[1] ; Biagi, Carlos A. ^[2] ; Silva Junior, Wilson A. ^[2] ; Zambuzzi, Willian F. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Lab Bioassays & Cellular Dynam, Dept Chem & Biochem, Inst Biosci, Campus Botucatu, Rua Prof Dr Irina Delanova, Botucatu, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Genom Med Ctr, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Materials Research Part A; v. 109, n. 6 SEP 2020.
• Citações Web of Science: 0

Resumo

Two in silico methodologies were implemented to reveal the molecular signatures of inorganic hydroxyapatite and beta-TCP materials from a transcriptome database to compare biomaterials. To test this new methodology, we choose the array E-MTAB-7219, which contains the transcription profile of osteoblastic cell line seeded onto 15 different biomaterials up to 48 hr. The expansive potential of the methodology was tested from the construction of customized signatures. We present, for the first time, a methodology to compare the performance of different biomaterials using the transcriptome profile of the cell through the Gene set variation analysis (GSVA) score. To test this methodology, we implemented two methods based on MSigDB collections, using all the collections and sub-collections except the Hallmark collection, which was used in the second method. The result of this analysis provided an initial understanding of biomaterial grouping based on the cell transcriptional landscape. The comparison using GSVA score combined efforts and expand the potential to compare biomaterials using transcriptome profile. Altogether, our results provide a better understanding of the comparison of different biomaterials and suggest a possibility of the new methodology be applied to the prospection of new biomaterials. (AU)

• Processo FAPESP: 14/22689-3 - Sinalização parácrina mediada por microvesículas e proteínas entre células ósseas e endoteliais durante o desenvolvimento e regeneração do tecido ósseo
• Beneficiário: Willian Fernando Zambuzzi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/05731-7 - PRP liofilizado associado à nano-hidroxiapatita na performance celular e regeneração óssea
• Beneficiário: Marcel Rodrigues Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/03639-8 - Mecanismos globais de transdução de sinais envolvidos com a resposta imediata a duas superfícies de ligas de titânio: construindo a base biodados OsteoBLAST
• Beneficiário: Marcel Rodrigues Ferreira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs